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PMID: 16789193 Published · ppublish English Journal Article

Post-Translational Proteolytic Cleavage of In Vitro-Synthesized Turnip Yellow Mosaic Virus RNA-Coded High-Molecular-Weight Proteins.

Journal of virology ·Vol. 34 ·No. 1 ·1980-04-00 ·Pages 85-94

Morch MD, Benicourt C

Abstract

In a reticulocyte lysate, turnip yellow mosaic virus genomic RNA directs the synthesis of two proteins with molecular weights of 150,000 (150K) and 195K. We present evidence that the larger protein is processed in vitro, after its completion, in at least three fragments. The NH(2)-terminal fragment (82K) and the COOH-terminal fragment (78K) have been well characterized by different methods. The fact that the 150K protein is not cleaved in vitro, although it contains the regions that are processed in the 195K protein, could be of fundamental biological significance for the expression of the viral genes: a single polypeptide chain could be processed in several ways, leading to different peptides with distinct biological activities.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Morch M D
Laboratoire de Biochimie du Développement, Institut de Recherches en Biologie Moléculaire, Université de Paris VII, 75221 Paris Cedex 05, France.
Benicourt C
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.34.1.85-94.1980
Published
1980-04-00
Pages
85-94
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288673
External Links
PubMed source DOI: 10.1128/JVI.34.1.85-94.1980 PMC Full Text View journal details
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