Home LiteratureArticle Details
PMID: 16789197 Published · ppublish English Journal Article

Genetic Analysis of Orbiviruses by Using RNase T(1) Oligonucleotide Fingerprints.

Journal of virology ·Vol. 34 ·No. 3 ·1980-06-00 ·Pages 583-91

Walker PJ, Mansbridge JN, Gorman BM

Abstract

Corresponding double-stranded RNA segments of the related orbiviruses Wallal and Mudjinbarry produced distinctly different RNase T(1) fingerprint patterns. No extensive sequence reiteration was observed between segments of Mudjinbarry virus. Fingerprint analysis of the genome of recombinant orbiviruses confirmed segment reassortment as a mechanism of interchange of genetic information. When temperature-sensitive mutants of each virus were crossed in mixed infection, a consistent pattern of segment reassortment was correlated with generation of the wild-type phenotype. Thus, the temperature-sensitive lesion of group II Wallal serogroup mutants was mapped to segment 1. The group I mutant lesion appears to be located in segment 2.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Walker P J
Queensland Institute of Medical Research, Bramston Terrace, Herston, 4006, Australia.
Mansbridge J N
Gorman B M
References (32)
32 references, click to expand
  1. "Fingerprinting" high molecular weight RNA by two-dimensional gel electrophoresis: application to poliovirus RNA.
    Nucleic Acids Res. 1976 Jul;3(7):1647-58 PMID: 184435
  2. The isolation and preliminary genetic classification of temperature-sensitive mutants of bluetongue virus.
    Onderstepoort J Vet Res. 1976 Dec;43(4):189-92 PMID: 191777
  3. The isolation of recombinants between related orbiviruses.
    J Gen Virol. 1978 Nov;41(2):333-42 PMID: 722283
  4. The RNA genome of Tilligerry virus.
    Aust J Exp Biol Med Sci. 1978 Jun;56(3):369-71 PMID: 708325
  5. A genetic map of reovirus. II. Assignment of the double-stranded RNA-negative mutant groups C, D, and E to genome segments.
    Virology. 1978 Apr;85(2):531-4 PMID: 664214
  6. A genetic map of reovirus. 1. Correlation of genome RNAs between serotypes 1, 2, and 3.
    Virology. 1978 Jan;84(1):63-74 PMID: 619493
  7. Formation of recombinants between snowshoe hare and La Crosse bunyaviruses.
    J Virol. 1977 Dec;24(3):893-902 PMID: 592468
  8. A provisional classification of cytoplasmic polyhedrosis viruses based on the sizes of the RNA genome segments.
    J Gen Virol. 1976 Oct;33(1):71-85 PMID: 789818
  9. Differentiation of human and calf reoviruslike agents associated with diarrhea using polyacrylamide gel electrophoresis of RNA.
    Virology. 1976 Oct 1;74(1):86-92 PMID: 988670
  10. Structure of the bluetongue virus capsid.
    J Virol. 1972 Oct;10(4):783-94 PMID: 4117349
  11. Molecular hybridization studies on the relationships between different serotypes of bluetongue virus and on the difference between the virulent and attenuated strains of the same serotype.
    Onderstepoort J Vet Res. 1973 Sep;40(3):93-103 PMID: 4132922
  12. Isolation of arboviruses from mosquitoes, biting midges, sandflies and vertebrates collected in Queensland, 1969 and 1970.
    Trans R Soc Trop Med Hyg. 1973;67(4):536-43 PMID: 4150461
  13. Comparison of the capsid polypeptides of various bluetongue virus serotypes.
    Intervirology. 1974;3(1-2):47-53 PMID: 4373408
  14. Thermal elution chromatography and the resolution of nucleic acids on hydroxylapatite.
    Anal Biochem. 1974 Sep;61(1):144-54 PMID: 4213501
  15. Mapping of the influenza virus genome: identification of the hemagglutinin and the neuraminidase genes.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):2142-6 PMID: 1064882
  16. Preparative two-dimensional polyacrylamide gel electrophoresis of 32 P-labeled RNA.
    Anal Biochem. 1972 Sep;49(1):184-97 PMID: 5081097
  17. A simple method for extraction of RNA from E. coli utilizing diethyl pyrocarbonate.
    Anal Biochem. 1970 Feb;33(2):459-63 PMID: 4910776
  18. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.
    Biochem J. 1967 Jan;102(1):251-7 PMID: 5339944
  19. A convenient procedure for preparing transfer ribonucleic acid from Escherichia coli.
    Biochim Biophys Acta. 1969 Apr 22;179(2):297-307 PMID: 4890595
  20. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  21. Isolation of intact individual species of single- and double-stranded RNA after fractionation by polyacrylamide gel electrophoresis.
    Anal Biochem. 1975 May 12;65(1-2):331-45 PMID: 1130684
  22. Purification and properties of the replicative intermediate of the RNA bacteriophage R17.
    Proc Natl Acad Sci U S A. 1966 Jun;55(6):1504-11 PMID: 5227669
  23. Polymorphism of the migration of double-stranded RNA genome segments of reovirus isolates from humans, cattle, and mice.
    J Virol. 1979 Jul;31(1):104-11 PMID: 501793
  24. Molecular weights of RNA segments of influenza A and B viruses.
    Virology. 1978 Jul 15;88(2):394-9 PMID: 695330
  25. Ecology of influenza viruses in lower mammals and birds.
    Br Med Bull. 1979 Jan;35(1):29-33 PMID: 367482
  26. Identification of the defective genes in three mutant groups of influenza virus.
    J Virol. 1977 Mar;21(3):1196-204 PMID: 850307
  27. Electrophoretic separation of double-stranded RNA genome segments from Warrego and Mitchell River viruses.
    Arch Virol. 1977;54(1-2):153-8 PMID: 196574
  28. Variation in orbiviruses.
    J Gen Virol. 1979 Jul;44(1):1-15 PMID: 91662
  29. Comparison of the genomes of simian, bovine, and human rotaviruses by gel electrophoresis and detection of genomic variation among bovine isolates.
    J Virol. 1979 Jun;30(3):839-46 PMID: 225545
  30. Protein synthesis in bluetongue virus-infected cells.
    Virology. 1979 Jan 30;92(2):385-96 PMID: 218351
  31. Comparison of human and animal rotavirus strains by gel electrophoresis of viral RNA.
    Virology. 1978 Jun 15;87(2):247-55 PMID: 208274
  32. The properties of two recent isolates of cytoplasmic polyhedrosis viruses.
    Intervirology. 1977;8(3):155-63 PMID: 323189
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.34.3.583-591.1980
Published
1980-06-00
Pages
583-91
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288745
External Links
PubMed source DOI: 10.1128/JVI.34.3.583-591.1980 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2