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PMID: 16789259 Published · ppublish English Journal Article

Complete Sequence and Enhancer Function of the Homologous DNA Regions of Autographa californica Nuclear Polyhedrosis Virus.

Journal of virology ·Vol. 60 ·No. 1 ·1986-10-00 ·Pages 224-9

Guarino LA, Gonzalez MA, Summers MD

Abstract

The nucleotide sequence of the five regions of homologous DNA in the genome of Autographa californica nuclear polyhedrosis virus DNA was determined. The homology of repeated sequences within a region was 65 to 87%, and the consensus sequences for each region were 88% homologous to each other. Sequences proximal to the EcoRI sites were most conserved, while the distal sequences were least conserved. The EcoRI sites formed the core of a 28-base-pair imperfect inverted repeat. All homologous regions functioned as enhancers in a transient expression assay. A single EcoRI minifragment located between EcoRI-Q and -L enhanced the expression of 39CAT as efficiently as the regions containing numerous EcoRI repeats did.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Guarino L A
Department of Entomology, Texas A&M University, and Texas Agricultural Experiment Station, College Station, Texas 77843-2475.
Gonzalez M A
Summers M D
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.60.1.224-229.1986
Published
1986-10-00
Pages
224-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253920
External Links
PubMed source DOI: 10.1128/JVI.60.1.224-229.1986 PMC Full Text View journal details
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