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PMID: 16789258 Published · ppublish English Journal Article

Interspersed Homologous DNA of Autographa californica Nuclear Polyhedrosis Virus Enhances Delayed-Early Gene Expression.

Journal of virology ·Vol. 60 ·No. 1 ·1986-10-00 ·Pages 215-23

Guarino LA, Summers MD

Abstract

The five regions of homologous DNA which are interspersed in the genome of the baculovirus Autographa californica nuclear polyhedrosis virus increased the expression of a delayed-early gene of this virus. Although this activity was first observed as a 10-fold trans effect, the homologous region 5 (hr5) enhanced the expression of linked genes 1,000-fold. The hr5 enhancer also exhibited the other characteristics associated with viral enhancer elements, including orientation independence and the abilities to function at a distance from the linked promoter, to regulate heterologous promoters, and to increase the number of RNA polymerase molecules transcribing the linked genes. The expression of the immediate-early regulatory gene was not enhanced by cis-linked hr5, although the enhancer function may require the immediate-early regulatory gene product. The hr5 enhancer was relatively insensitive to competition by an excess of enhancer molecules. The nucleotide sequence of hr5 revealed two different conserved repeats separated by nonhomologous DNA. Deletion analysis of the hr5 enhancer indicated that a 30-base-pair inverted repeat was essential for enhancer function.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Guarino L A
Department of Entomology, Texas A&M University, and Texas Agricultural Experiment Station, College Station, Texas 77843-2475.
Summers M D
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.60.1.215-223.1986
Published
1986-10-00
Pages
215-23
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253919
External Links
PubMed source DOI: 10.1128/JVI.60.1.215-223.1986 PMC Full Text View journal details
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