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PMID: 16822837 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The STE20/germinal center kinase POD6 interacts with the NDR kinase COT1 and is involved in polar tip extension in Neurospora crassa.

Molecular biology of the cell ·Vol. 17 ·No. 9 ·2006-09-00 ·Pages 4080-92

Seiler S, Vogt N, Ziv C, Gorovits R, Yarden O

Abstract

Members of the Ste20 and NDR protein kinase families are important for normal cell differentiation and morphogenesis in various organisms. We characterized POD6 (NCU02537.2), a novel member of the GCK family of Ste20 kinases that is essential for hyphal tip extension and coordinated branch formation in the filamentous fungus Neurospora crassa. pod-6 and the NDR kinase mutant cot-1 exhibit indistinguishable growth defects, characterized by cessation of cell elongation, hyperbranching, and altered cell-wall composition. We suggest that POD6 and COT1 act in the same genetic pathway, based on the fact that both pod-6 and cot-1 can be suppressed by 1) environmental stresses, 2) altering protein kinase A activity, and 3) common extragenic suppressors (ropy, as well as gul-1, which is characterized here as the ortholog of the budding and fission yeasts SSD1 and Sts5, respectively). Unlinked noncomplementation of cot-1/pod-6 alleles indicates a potential physical interaction between the two kinases, which is further supported by coimmunoprecipitation analyses, partial colocalization of both proteins in wild-type cells, and their common mislocalization in dynein/kinesin mutants. We conclude that POD6 acts together with COT1 and is essential for polar cell extension in a kinesin/dynein-dependent manner in N. crassa.

MeSH Terms
Amino Acid Sequence Catalytic Domain Cell Polarity/drug effects Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors Fungal Proteins/metabolism Germinal Center Kinases Hyphae/cytology Microtubules/metabolism Molecular Motor Proteins/metabolism Molecular Sequence Data Mutation/drug effects,genetics Neurospora crassa/cytology,drug effects,enzymology Phenotype Protein Binding/drug effects Protein Serine-Threonine Kinases/chemistry,metabolism Protein Transport/drug effects Sequence Alignment Sodium Chloride/pharmacology
Chemicals
Fungal Proteins Germinal Center Kinases Molecular Motor Proteins Sodium Chloride Protein Serine-Threonine Kinases Cyclic AMP-Dependent Protein Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Seiler Stephan
Deutsche Forschungsgemeinschaft Research Center of Molecular Physiology of the Brain (CMPB), Abteilung Molekulare Mikrobiologie, Universität Göttingen, D-37077 Göttingen, Germany. [email protected]
Vogt Nico
Ziv Carmit
Gorovits Rena
Yarden Oded
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e06-01-0072
Email
[email protected]
Published
2006-09-00
Epub
2006-00-05
Pages
4080-92
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1593175
Subset
IM
Databases
GENBANK
DQ336953
External Links
PubMed source DOI: 10.1091/mbc.e06-01-0072 PMC Full Text View journal details
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