Home LiteratureArticle Details
PMID: 16888139 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Regulation of sexual development of Plasmodium by translational repression.

Science (New York, N.Y.) ·Vol. 313 ·No. 5787 ·2006-08-04 ·Pages 667-9

Mair GR, Braks JA, Garver LS, Wiegant JC, Hall N, Dirks RW, Khan SM, Dimopoulos G, Janse CJ, Waters AP

Abstract

Translational repression of messenger RNAs (mRNAs) plays an important role in sexual differentiation and gametogenesis in multicellular eukaryotes. Translational repression and mRNA turnover were shown to influence stage-specific gene expression in the protozoan Plasmodium. The DDX6-class RNA helicase, DOZI (development of zygote inhibited), is found in a complex with mRNA species in cytoplasmic bodies of female, blood-stage gametocytes. These translationally repressed complexes are normally stored for translation after fertilization. Genetic disruption of pbdozi inhibits the formation of the ribonucleoprotein complexes, and instead, at least 370 transcripts are diverted to a degradation pathway.

MeSH Terms
Animals Crosses, Genetic Gene Expression Regulation, Developmental Gene Silencing Genes, Protozoan Oligonucleotide Array Sequence Analysis Plasmodium/enzymology,genetics Plasmodium berghei/enzymology,genetics,growth & development,physiology Protein Biosynthesis Protozoan Proteins/metabolism RNA Helicases/genetics,metabolism RNA, Messenger/metabolism RNA, Messenger, Stored/metabolism RNA, Protozoan/metabolism Recombinant Fusion Proteins/metabolism Ribonucleoproteins/metabolism
Chemicals
Protozoan Proteins RNA, Messenger RNA, Messenger, Stored RNA, Protozoan Recombinant Fusion Proteins Ribonucleoproteins RNA Helicases
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Mair Gunnar R
Department of Parasitology, Leiden University Medical Centre, 2333 ZA Leiden, Netherlands.
Braks Joanna A M
Garver Lindsey S
Wiegant Joop C A G
Hall Neil
Dirks Roeland W
Khan Shahid M
Dimopoulos George
Janse Chris J
Waters Andrew P
References (24)
24 references, click to expand
  1. Calcium and a calcium-dependent protein kinase regulate gamete formation and mosquito transmission in a malaria parasite.
    Cell. 2004 May 14;117(4):503-14 PMID: 15137943
  2. The DEAD-box protein family of RNA helicases.
    Gene. 2006 Feb 15;367:17-37 PMID: 16337753
  3. Movement of eukaryotic mRNAs between polysomes and cytoplasmic processing bodies.
    Science. 2005 Oct 21;310(5747):486-9 PMID: 16141371
  4. A conserved role of a DEAD box helicase in mRNA masking.
    RNA. 2001 Dec;7(12):1728-42 PMID: 11780630
  5. Structure and expression of a post-transcriptionally regulated malaria gene encoding a surface protein from the sexual stages of Plasmodium berghei.
    Mol Biochem Parasitol. 1993 Jun;59(2):263-75 PMID: 8341324
  6. Me31B silences translation of oocyte-localizing RNAs through the formation of cytoplasmic RNP complex during Drosophila oogenesis.
    Development. 2001 Sep;128(17):3233-42 PMID: 11546740
  7. Xp54, the Xenopus homologue of human RNA helicase p54, is an integral component of stored mRNP particles in oocytes.
    Nucleic Acids Res. 1997 Mar 1;25(5):965-73 PMID: 9023105
  8. General translational repression by activators of mRNA decapping.
    Cell. 2005 Sep 23;122(6):875-86 PMID: 16179257
  9. Proteome analysis of separated male and female gametocytes reveals novel sex-specific Plasmodium biology.
    Cell. 2005 Jun 3;121(5):675-87 PMID: 15935755
  10. In situ detection of Pbs21 mRNA during sexual development of Plasmodium berghei.
    Mol Biochem Parasitol. 1994 Dec;68(2):189-96 PMID: 7739665
  11. Differential expression in blood stages of the gene coding for the 21-kilodalton surface protein of ookinetes of Plasmodium berghei as detected by RNA in situ hybridisation.
    Mol Biochem Parasitol. 1994 Dec;68(2):259-66 PMID: 7739671
  12. Genome sequence of the human malaria parasite Plasmodium falciparum.
    Nature. 2002 Oct 3;419(6906):498-511 PMID: 12368864
  13. Analysis of the Plasmodium and Anopheles transcriptional repertoire during ookinete development and midgut invasion.
    J Biol Chem. 2004 Feb 13;279(7):5573-80 PMID: 14627712
  14. von Willebrand Factor A domain-related protein, a novel microneme protein of the malaria ookinete highly conserved throughout Plasmodium parasites.
    Mol Biochem Parasitol. 2001 Aug;116(1):65-72 PMID: 11463467
  15. Distinct roles for pbs21 and pbs25 in the in vitro ookinete to oocyst transformation of Plasmodium berghei.
    J Cell Sci. 2000 Oct;113 Pt 19:3419-26 PMID: 10984433
  16. The power of the 3' UTR: translational control and development.
    Nat Rev Genet. 2003 Aug;4(8):626-37 PMID: 12897774
  17. A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses.
    Science. 2005 Jan 7;307(5706):82-6 PMID: 15637271
  18. A central role for P48/45 in malaria parasite male gamete fertility.
    Cell. 2001 Jan 12;104(1):153-64 PMID: 11163248
  19. Constraints on transcriptional activator function contribute to transcriptional quiescence during early Xenopus embryogenesis.
    EMBO J. 1995 Apr 18;14(8):1752-65 PMID: 7737126
  20. The DEAD box helicase, Dhh1p, functions in mRNA decapping and interacts with both the decapping and deadenylase complexes.
    RNA. 2001 Dec;7(12):1717-27 PMID: 11780629
  21. Decapping and decay of messenger RNA occur in cytoplasmic processing bodies.
    Science. 2003 May 2;300(5620):805-8 PMID: 12730603
  22. Localization of ribosomal RNA and Pbs21-mRNA in the sexual stages of Plasmodium berghei using electron microscope in situ hybridization.
    Eur J Cell Biol. 1996 Nov;71(3):270-6 PMID: 8929565
  23. Characterisation and expression of pbs25, a sexual and sporogonic stage specific protein of Plasmodium berghei.
    Mol Biochem Parasitol. 2000 Sep;110(1):147-59 PMID: 10989152
  24. RNA-binding proteins in early development.
    Crit Rev Biochem Mol Biol. 2005 Jan-Feb;40(1):21-73 PMID: 15804624
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
DOI
10.1126/science.1125129
Published
2006-08-04
Pages
667-9
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC1609190
Subset
IM
Grants
Wellcome Trust · 066742 · United Kingdom
NIAID NIH HHS · 1R01AI061576-01A1 · United States
Databases
GEO
GSM111868, GSM111871, GSM111872, GSM111873, GSM111874, GSM111875
Corrections
CommentIn
PMID: 16888130
External Links
PubMed source DOI: 10.1126/science.1125129 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2