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PMID: 16893473 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Identification of differentially expressed genes in cutaneous squamous cell carcinoma by microarray expression profiling.

Molecular cancer ·Vol. 5 ·2006-08-08 ·Pages 30

Nindl I, Dang C, Forschner T, Kuban RJ, Meyer T, Sterry W, Stockfleth E

Abstract

Carcinogenesis is a multi-step process indicated by several genes up- or down-regulated during tumor progression. This study examined and identified differentially expressed genes in cutaneous squamous cell carcinoma (SCC). Three different biopsies of 5 immunosuppressed organ-transplanted recipients each normal skin (all were pooled), actinic keratosis (AK) (two were pooled), and invasive SCC and additionally 5 normal skin tissues from immunocompetent patients were analyzed. Thus, total RNA of 15 specimens were used for hybridization with Affymetrix HG-U133A microarray technology containing 22,283 genes. Data analyses were performed by prediction analysis of microarrays using nearest shrunken centroids with the threshold 3.5 and ANOVA analysis was independently performed in order to identify differentially expressed genes (p < 0.05). Verification of 13 up- or down-regulated genes was performed by quantitative real-time reverse transcription (RT)-PCR and genes were additionally confirmed by sequencing. Broad coherent patterns in normal skin vs. AK and SCC were observed for 118 genes. The majority of identified differentially expressed genes in cutaneous SCC were previously not described.

MeSH Terms
Adolescent Aged Carcinoma, Squamous Cell/genetics Female Gene Expression Profiling Humans Male Middle Aged Multigene Family/genetics Oligonucleotide Array Sequence Analysis RNA, Messenger/genetics Skin Neoplasms/genetics
Chemicals
RNA, Messenger
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Nindl Ingo
Department of Dermatology, Charité, Skin Cancer Center Charité, University Hospital of Berlin, Charitéplatz 1, D-10117 Berlin, Germany. [email protected]
Dang Chantip
Forschner Tobias
Kuban Ralf J
Meyer Thomas
Sterry Wolfram
Stockfleth Eggert
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Article Info
Journal
Molecular cancer
Abbr.
Mol Cancer
ISSN
1476-4598
DOI
10.1186/1476-4598-5-30
Email
[email protected]
Published
2006-08-08
Epub
2006-00-08
Pages
30
Language
English
Region
England
NLM ID
101147698
PMCID
PMC1569867
Subset
IM
External Links
PubMed source DOI: 10.1186/1476-4598-5-30 PMC Full Text View journal details
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