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PMID: 1690391 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Infectious DNA of the human spumaretrovirus.

Nucleic acids research ·Vol. 18 ·No. 4 ·1990-02-25 ·Pages 733-8

Rethwilm A, Baunach G, Netzer KO, Maurer B, Borisch B, ter Meulen V

Abstract

An infectious molecular clone (pHSRV) of the human Spumaretrovirus (HSRV) was constructed using viral DNA and cDNA clones. The infectivity of pHSRV was proven by transfection of cell cultures and subsequent infection of susceptible cultures with cell free transfection derived virus. pHSRV derived virus produced foamy virus typical cytopathic effects in susceptible cultures. Infected cells could be stained specifically with foamy virus antisera by means of indirect immunofluorescence. Radioimmunoprecipitation revealed the presence of characteristic HSRV structural proteins in pHSRV infected cultures. By cotransfection of pHSRV and an indicator plasmid it was found that pHSRV is able to transactivate the viral LTR. Viral transcripts were found to be approximately 200 bases longer in pHSRV infected cultures compared to wildtype infected cultures. This difference is most likely due to an insertion of DNA of non-viral origin in the U3 region of the 3'LTR of the infectious clone.

MeSH Terms
Animals Blotting, Northern Cell Line Cell Transformation, Viral Cloning, Molecular DNA, Viral/genetics Genes, Viral Humans Nucleic Acid Hybridization Plasmids Poly A/genetics,isolation & purification RNA/genetics,isolation & purification RNA, Messenger RNA, Viral/genetics,isolation & purification Retroviridae/genetics Transcription, Genetic Transfection
Chemicals
DNA, Viral RNA, Messenger RNA, Viral Poly A RNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rethwilm A
Institut für Virologie, Universität Würzburg, FRG.
Baunach G
Netzer K O
Maurer B
Borisch B
ter Meulen V
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.4.733
Published
1990-02-25
Pages
733-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC330320
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/18.4.733 PMC Full Text View journal details
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