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PMID: 2820721 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence analysis of the env gene and its flanking regions of the human spumaretrovirus reveals two novel genes.

The EMBO journal ·Vol. 6 ·No. 7 ·1987-07-00 ·Pages 2077-84

Flügel RM, Rethwilm A, Maurer B, Darai G

Abstract

Recombinant clones that represent the 3' part of the genome of the human spumaretrovirus (foamy virus) were established from viral DNA and from DNA complementary to viral RNA. The recombinant clones were characterized by blot hybridizations and nucleotide sequence analysis. The deduced protein sequence of the clones at their 5' ends was found to be homologous to the 3' domain of retroviral reverse transcriptases. Downstream of a small intergenic pol-env region a long open reading frame of 985 amino acid residues was identified that according to its genomic location, size, glycosylation signals, and hydrophobicity profile closely resembles the lentiviral env genes. The spumaretroviral env gene is followed by two open reading frames, termed bel-1 and bel-2 which are located between env and the long terminal repeat region. The long terminal repeat of 1259 nucleotides is preceded by a polypurine tract and contains the canonical signal sequences characteristic for transcriptional regulation of retroviruses. The provisional classification of the spumaretrovirus subfamily is discussed.

MeSH Terms
Amino Acid Sequence Base Sequence Cells, Cultured Cloning, Molecular DNA Restriction Enzymes Genes Genes, Viral Humans Molecular Sequence Data Plasmids Retroviridae/genetics Spumavirus/genetics Viral Envelope Proteins/genetics
Chemicals
Viral Envelope Proteins DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Flügel R M
Institute for Virus Research, German Cancer Research Center, Heidelberg.
Rethwilm A
Maurer B
Darai G
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42 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-07-00
Pages
2077-84
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553598
Subset
IM
Databases
GENBANK
X05591, X05592, Y00070
Corrections
ErratumIn
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External Links
PubMed source PMC Full Text View journal details
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