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PMID: 3031462 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence analysis of a cloned DNA fragment from human cells reveals homology to retrotransposons.

Molecular and cellular biology ·Vol. 7 ·No. 1 ·1987-01-00 ·Pages 231-6

Flügel RM, Maurer B, Bannert H, Rethwilm A, Schnitzler P, Darai G

Abstract

During molecular cloning of proviral DNA of human spumaretrovirus, various recombinant clones were established and analyzed. Blot hybridization revealed that one of the recombinant plasmids had the characteristic features of a member of the long interspersed repetitive sequences family. The DNA element was analyzed by restriction mapping and nucleotide sequencing. It showed a high degree of amino acid sequence homology of 54.3% when compared with the 5'-terminal part of the pol gene product of the murine retrotransposon LIMd. The 3' region of the cloned DNA element encodes proteins with an even higher degree of homology of 67.4% in comparison to the corresponding parts of a member of the primate KpnI sequence family.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Line Cloning, Molecular DNA/analysis DNA Restriction Enzymes DNA Transposable Elements Humans Lung/embryology Retroviridae/genetics Sequence Homology, Nucleic Acid
Chemicals
DNA Transposable Elements DNA DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Flügel R M
Maurer B
Bannert H
Rethwilm A
Schnitzler P
Darai G
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23 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.1.231-236.1987
Published
1987-01-00
Pages
231-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365061
Subset
IM
Databases
GENBANK
M16031
External Links
PubMed source DOI: 10.1128/mcb.7.1.231-236.1987 PMC Full Text View journal details
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