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PMID: 1691614 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Homology of mecA gene in methicillin-resistant Staphylococcus haemolyticus and Staphylococcus simulans to that of Staphylococcus aureus.

Antimicrobial agents and chemotherapy ·Vol. 34 ·No. 1 ·1990-01-00 ·Pages 170-2

Ubukata K, Nonoguchi R, Song MD, Matsuhashi M, Konno M

Abstract

A penicillin-binding protein of molecular weight 76,000 inducible by beta-lactams was detected in methicillin-resistant Staphylococcus haemolyticus and Staphylococcus simulans. DNA from these strains hybridized to the mecA gene from Staphylococcus aureus; however, the chromosomal HindIII fragments containing the mecA genes were 3.4 kilobases in S. haemolyticus and 4.3 kilobases in S. simulans.

MeSH Terms
Bacterial Proteins Blotting, Southern Carrier Proteins/analysis,isolation & purification Culture Media DNA, Bacterial/metabolism Deoxyribonuclease HindIII/analysis Electrophoresis, Polyacrylamide Gel Gene Expression Regulation, Bacterial Genes, Bacterial Hexosyltransferases Methicillin/pharmacology Muramoylpentapeptide Carboxypeptidase/analysis,isolation & purification Nucleic Acid Hybridization Penicillin Resistance Penicillin-Binding Proteins Peptidyl Transferases Restriction Mapping Staining and Labeling Staphylococcus/drug effects,genetics Staphylococcus aureus/drug effects,genetics
Chemicals
Bacterial Proteins Carrier Proteins Culture Media DNA, Bacterial Penicillin-Binding Proteins Peptidyl Transferases Hexosyltransferases Deoxyribonuclease HindIII Muramoylpentapeptide Carboxypeptidase Methicillin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ubukata K
Department of Clinical Pathology, Teikyo University School of Medicine, Tokyo, Japan.
Nonoguchi R
Song M D
Matsuhashi M
Konno M
References (14)
14 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.34.1.170
Published
1990-01-00
Pages
170-2
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC171544
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.34.1.170 PMC Full Text View journal details
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