Home LiteratureArticle Details
PMID: 3848293 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of penicillin-binding protein activity: description of a methicillin-inducible penicillin-binding protein in Staphylococcus aureus.

Antimicrobial agents and chemotherapy ·Vol. 27 ·No. 5 ·1985-05-00 ·Pages 828-31

Rossi L, Tonin E, Cheng YR, Fontana R

Abstract

The penicillin-binding proteins (PBPs) of two methicillin-resistant strains of Staphylococcus aureus (R2 and R1) were analyzed in cells grown in the absence and in the presence of methicillin. Under the former condition, strain R2 showed the typical PBP pattern of beta-lactam-susceptible strains, while strain R1 showed a markedly increased amount of PBP-3. Under the latter condition, on the other hand, a novel PBP (PBP-2a) located between PBP-2 and -3 was detected in strain R2, while strain R1 appeared to synthesize an even greater amount of PBP-3, in respect to untreated cells. Both R2 PBP-2a and R1 PBP-3 showed a very low affinity for methicillin, which was consistent with the MICs for the respective strains.

MeSH Terms
Bacterial Proteins/analysis Binding, Competitive/drug effects Carboxypeptidases/metabolism Carrier Proteins/metabolism Hexosyltransferases Methicillin/pharmacology Microbial Sensitivity Tests Muramoylpentapeptide Carboxypeptidase/metabolism Penicillin Resistance Penicillin-Binding Proteins Peptidyl Transferases Staphylococcus aureus/drug effects,metabolism
Chemicals
Bacterial Proteins Carrier Proteins Penicillin-Binding Proteins Peptidyl Transferases Hexosyltransferases Carboxypeptidases Muramoylpentapeptide Carboxypeptidase Methicillin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rossi L
Tonin E
Cheng Y R
Fontana R
References (19)
19 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate.
    Antimicrob Agents Chemother. 1972 Apr;1(4):283-8 PMID: 4208895
  3. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  4. Interaction of penicillin with the bacterial cell: penicillin-binding proteins and penicillin-sensitive enzymes.
    Bacteriol Rev. 1974 Sep;38(3):291-335 PMID: 4608953
  5. Penicillin-binding proteins and cell shape in E. coli.
    Nature. 1975 Apr 10;254(5500):516-7 PMID: 1091862
  6. Clavulanic acid: a beta-lactamase-inhiting beta-lactam from Streptomyces clavuligerus.
    Antimicrob Agents Chemother. 1977 May;11(5):852-7 PMID: 879738
  7. The mechanism of the irreversible antimicrobial effects of penicillins: how the beta-lactam antibiotics kill and lyse bacteria.
    Annu Rev Microbiol. 1979;33:113-37 PMID: 40528
  8. Identification of the lethal target of benzylpenicillin in Streptococcus faecalis by in vivo penicillin binding studies.
    Nature. 1980 Sep 4;287(5777):70-2 PMID: 6774266
  9. Intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus.
    FEBS Lett. 1980 Dec 29;122(2):275-8 PMID: 7202719
  10. Membrane-bound penicillinases in Gram-positive bacteria.
    J Biol Chem. 1982 Apr 25;257(8):4490-5 PMID: 6802832
  11. Synthesis of penicillin-binding protein 6 by stationary-phase Escherichia coli.
    J Bacteriol. 1982 Jul;151(1):491-4 PMID: 7045084
  12. Penicillin-binding proteins in a Staphylococcus aureus strain resistant to specific beta-lactam antibiotics.
    Antimicrob Agents Chemother. 1982 Jul;22(1):172-5 PMID: 7125630
  13. Alteration in the penicillin-binding profile of Bacillus megaterium during sporulation.
    Nature. 1982 Dec 16;300(5893):640-3 PMID: 6815541
  14. Synthesis of peptidoglycan in vivo in methicillin-resistant Staphylococcus aureus.
    Eur J Biochem. 1982 Oct;127(3):553-8 PMID: 7173195
  15. Streptococcus faecium ATCC 9790 penicillin-binding proteins and penicillin sensitivity are heavily influenced by growth conditions: proposal for an indirect mechanism of growth inhibition by beta-lactams.
    J Bacteriol. 1983 May;154(2):916-23 PMID: 6841320
  16. Identification of a streptococcal penicillin-binding protein that reacts very slowly with penicillin.
    J Bacteriol. 1983 Sep;155(3):1343-50 PMID: 6411688
  17. Penicillin-binding proteins and the mechanism of action of beta-lactam antibiotics.
    Annu Rev Biochem. 1983;52:825-69 PMID: 6351730
  18. Penicillin-binding proteins and the future of beta-lactam antibiotics. The Seventh Fleming Lecture.
    J Gen Microbiol. 1983 May;129(5):1247-60 PMID: 6352855
  19. Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus.
    J Bacteriol. 1984 May;158(2):513-6 PMID: 6563036
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.27.5.828
Published
1985-05-00
Pages
828-31
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC180160
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.27.5.828 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2