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PMID: 1692413 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA-protein interactions at the interferon-responsive promoter: evidences for an involvement of phosphorylation.

Nucleic acids research ·Vol. 18 ·No. 8 ·1990-04-25 ·Pages 2125-31

Roy C, Lebleu B

Abstract

Several alpha beta and gamma interferons (IFN) induced genes are regulated transcriptionally via a 29 bp cis acting regulatory element (ICS, Interferon Consensus Sequence). The ICS binding capacity of HeLa cell nuclear extracts was strictly dependent on the presence of nucleoside triphosphate and Mg2+. It increased upon alpha beta or gamma IFN treatment of the cells. Three 50 mer synthetic oligonucleotides containing the ICS or putative ISRE (Interferon Stimulatable Response Element), representing portions of genes inducible by alpha beta IFN (HLA-A3 and IFI-56K) or by gamma (HLA-DR) were used as probes to titrate nuclear factors interacting with the ICS. All three probes were retarded in a mobility shift assay in two bands. Phosphorylation conditions were crucial for demonstrating their existence and/or their relative amounts. A factor whose activity and/or amount was modulated upon IFN treatment, appeared to be involved in phosphorylation dependent post-translational modification(s) of the ISRE binding proteins responsible for altered binding properties.

MeSH Terms
Adenosine Triphosphate/metabolism Base Sequence DNA/genetics,metabolism DNA-Binding Proteins/metabolism HeLa Cells Humans Interferons/pharmacology Magnesium/pharmacology Molecular Sequence Data Phosphorylation Promoter Regions, Genetic
Chemicals
DNA-Binding Proteins Adenosine Triphosphate DNA Interferons Magnesium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Roy C
UA CNRS 1191, Laboratoire de Biochimie des Protéines, Université de Montpellier II, France.
Lebleu B
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33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.8.2125
Published
1990-04-25
Pages
2125-31
Language
English
Region
England
NLM ID
0411011
PMCID
PMC330692
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/18.8.2125 PMC Full Text View journal details
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