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PMID: 1692961 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A growth arrest-specific (gas) gene codes for a membrane protein.

Molecular and cellular biology ·Vol. 10 ·No. 6 ·1990-06-00 ·Pages 2924-30

Manfioletti G, Ruaro ME, Del Sal G, Philipson L, Schneider C

Abstract

A set of growth arrest-specific (gas) genes whose expression is negatively regulated by serum has recently been identified. We report on the detailed analysis of one of these genes (gas3). The kinetics of regulation by the presence and absence of serum were investigated, and it was found that this gene is regulated at the post-transcriptional level. The encoded protein deduced from the nucleotide sequence showed some similarity to a mitochondrial oxyreductase, and in vitro translation established that the protein product is a transmembrane glycoprotein.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Cattle Cell Division Cells, Cultured Cloning, Molecular Culture Media DNA/genetics,isolation & purification DNA Replication Gene Library Genes Kinetics Macromolecular Substances Membrane Proteins/genetics Mice Molecular Sequence Data NAD(P)H Dehydrogenase (Quinone) Protein Biosynthesis Quinone Reductases/genetics RNA/isolation & purification RNA, Messenger/biosynthesis,genetics Rabbits Reticulocytes/metabolism Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
Culture Media Macromolecular Substances Membrane Proteins RNA, Messenger RNA DNA NAD(P)H Dehydrogenase (Quinone) Quinone Reductases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Manfioletti G
International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
Ruaro M E
Del Sal G
Philipson L
Schneider C
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25 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.6.2924-2930.1990
Published
1990-06-00
Pages
2924-30
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360655
Subset
IM
Databases
GENBANK
M32240
External Links
PubMed source DOI: 10.1128/mcb.10.6.2924-2930.1990 PMC Full Text View journal details
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