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PMID: 1694128 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Control of replication of plasmid R1: the duplex between the antisense RNA, CopA, and its target, CopT, is processed specifically in vivo and in vitro by RNase III.

The EMBO journal ·Vol. 9 ·No. 7 ·1990-07-00 ·Pages 2331-40

Blomberg P, Wagner EG, Nordström K

Abstract

The replication frequency of IncFII plasmids is regulated through the availability of a rate-limiting protein, RepA. The synthesis of this protein is controlled post-transcriptionally by a small antisense RNA, CopA, which binds to the leader region of the RepA mRNA (CopT). In this communication we report studies of the IncFII plasmid R1. We show that the duplex between CopA and CopT is cleaved specifically in vivo. The in vivo cleavage maps to the same position as that resulting from in vitro cleavage of a CopA/CopT duplex by purified RNase III. By introducing plasmids carrying translational repA-lacZ fusions into cells deficient in RNase III we show that the expression of repA is elevated when RNase III activity is severely decreased. Hence, cleavage by RNase III seems to be a key event in the copy number control system of plasmid R1.

MeSH Terms
Bacterial Proteins/metabolism Base Sequence DNA Helicases DNA Replication DNA-Binding Proteins Endoribonucleases/metabolism Escherichia coli/genetics,metabolism Escherichia coli Proteins Genotype Molecular Sequence Data Nucleotide Mapping Oligonucleotide Probes Proteins R Factors RNA/genetics RNA Probes RNA Processing, Post-Transcriptional RNA, Antisense RNA, Messenger/antagonists & inhibitors,genetics Restriction Mapping Ribonuclease III Single-Strand Specific DNA and RNA Endonucleases Trans-Activators
Chemicals
Bacterial Proteins DNA-Binding Proteins Escherichia coli Proteins Oligonucleotide Probes Proteins RNA Probes RNA, Antisense RNA, Messenger Trans-Activators replication initiator protein RNA Endoribonucleases Ribonuclease III ribonuclease III, E coli Single-Strand Specific DNA and RNA Endonucleases DNA Helicases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Blomberg P
Department of Microbiology, Uppsala University, Sweden.
Wagner E G
Nordström K
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37 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-07-00
Pages
2331-40
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551961
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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