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PMID: 16950127 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The FERM protein Yurt is a negative regulatory component of the Crumbs complex that controls epithelial polarity and apical membrane size.

Developmental cell ·Vol. 11 ·No. 3 ·2006-09-00 ·Pages 363-74

Laprise P, Beronja S, Silva-Gagliardi NF, Pellikka M, Jensen AM, McGlade CJ, Tepass U

Abstract

The Crumbs (Crb) complex is a key regulator of epithelial cell architecture where it promotes apical membrane formation. Here, we show that binding of the FERM protein Yurt to the cytoplasmic domain of Crb is part of a negative-feedback loop that regulates Crb activity. Yurt is predominantly a basolateral protein but is recruited by Crb to apical membranes late during epithelial development. Loss of Yurt causes an expansion of the apical membrane in embryonic epithelia and photoreceptor cells similar to Crb overexpression and in contrast to loss of Crb. Analysis of yurt crb double mutants suggests that these genes function in one pathway and that yurt negatively regulates crb. We also show that the mammalian Yurt orthologs YMO1 and EHM2 bind to mammalian Crb proteins. We propose that Yurt is part of an evolutionary conserved negative-feedback mechanism that restricts Crb complex activity in promoting apical membrane formation.

MeSH Terms
Animals Body Patterning Cell Membrane/physiology Cell Polarity Drosophila Proteins/genetics,metabolism,physiology Drosophila melanogaster/embryology Embryonic Induction/genetics Epithelium/physiology Humans Membrane Proteins/metabolism,physiology Mice Retina/embryology Spinal Cord/embryology
Chemicals
Drosophila Proteins Membrane Proteins crb protein, Drosophila yrt protein, Drosophila
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Laprise Patrick
Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario M5S 3G5, Canada.
Beronja Slobodan
Silva-Gagliardi Nancy F
Pellikka Milena
Jensen Abbie M
McGlade C Jane
Tepass Ulrich
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Article Info
Journal
Developmental cell
Abbr.
Dev Cell
ISSN
1534-5807
DOI
10.1016/j.devcel.2006.06.001
Published
2006-09-00
Pages
363-74
Language
English
Region
United States
NLM ID
101120028
PMCID
PMC2834949
Subset
IM
Grants
NEI NIH HHS · R01 EY015420-02 · United States
NEI NIH HHS · R01 EY015420-01 · United States
NEI NIH HHS · R01 EY015420-03 · United States
NEI NIH HHS · R01 EY015420-04 · United States
NEI NIH HHS · R01 EY015420 · United States
External Links
PubMed source DOI: 10.1016/j.devcel.2006.06.001 PMC Full Text View journal details
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