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PMID: 15238527 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes.

Genetics ·Vol. 167 ·No. 2 ·2004-06-00 ·Pages 761-81

Bellen HJ, Levis RW, Liao G, He Y, Carlson JW, Tsang G, Evans-Holm M, Hiesinger PR, Schulze KL, Rubin GM, Hoskins RA, Spradling AC

Abstract

The Berkeley Drosophila Genome Project (BDGP) strives to disrupt each Drosophila gene by the insertion of a single transposable element. As part of this effort, transposons in >30,000 fly strains were localized and analyzed relative to predicted Drosophila gene structures. Approximately 6300 lines that maximize genomic coverage were selected to be sent to the Bloomington Stock Center for public distribution, bringing the size of the BDGP gene disruption collection to 7140 lines. It now includes individual lines predicted to disrupt 5362 of the 13,666 currently annotated Drosophila genes (39%). Other lines contain an insertion at least 2 kb from others in the collection and likely mutate additional incompletely annotated or uncharacterized genes and chromosomal regulatory elements. The remaining strains contain insertions likely to disrupt alternative gene promoters or to allow gene misexpression. The expanded BDGP gene disruption collection provides a public resource that will facilitate the application of Drosophila genetics to diverse biological problems. Finally, the project reveals new insight into how transposons interact with a eukaryotic genome and helps define optimal strategies for using insertional mutagenesis as a genomic tool.

Keywords
Non-programmatic
MeSH Terms
Animals DNA Transposable Elements/genetics Drosophila/genetics Genes, Insect/genetics Genome Models, Genetic
Chemicals
DNA Transposable Elements
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Bellen Hugo J
Department of Molecular and Human Genetics, Howard Hughes Medical Institute, Program in Developmental Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
Levis Robert W
Liao Guochun
He Yuchun
Carlson Joseph W
Tsang Garson
Evans-Holm Martha
Hiesinger P Robin
Schulze Karen L
Rubin Gerald M
Hoskins Roger A
Spradling Allan C
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.104.026427
Published
2004-06-00
Pages
761-81
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1470905
Subset
IM
Grants
Howard Hughes Medical Institute · United States
External Links
PubMed source DOI: 10.1534/genetics.104.026427 PMC Full Text View journal details
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