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PMID: 11562481 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vivo transposition of Minos, a Drosophila mobile element, in mammalian tissues.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 20 ·2001-09-25 ·Pages 11474-8

Zagoraiou L, Drabek D, Alexaki S, Guy JA, Klinakis AG, Langeveld A, Skavdis G, Mamalaki C, Grosveld F, Savakis C

Abstract

Transposable elements have been used widely in the past 20 years for gene transfer and insertional mutagenesis in Drosophila. Transposon-based technology for gene manipulation and genomic analysis currently is being adopted for vertebrates. We tested the ability of Minos, a DNA transposon from Drosophila hydei, to transpose in mouse tissues. Two transgenic mouse lines were crossed, one expressing Minos transposase in lymphocytes under the control of the CD2 promoter/locus control region and another carrying a nonautonomous Minos transposon. Only mice containing both transgenes show excision of the transposon and transposition into new chromosomal sites in thymus and spleen cells. In addition, expression of Minos transposase in embryonic fibroblast cell lines derived from a transposon-carrying transgenic mouse resulted in excision of the transposon. These results are a first step toward a reversible insertional mutagenesis system in the mouse, opening the way to develop powerful technologies for functional genomic analysis in mammals.

MeSH Terms
Animals Base Sequence Cell Line Chromosome Mapping Drosophila/embryology,genetics Female Male Mice Mice, Transgenic Mutagenesis, Insertional Spleen/enzymology Telomere/genetics Thymus Gland/enzymology Transfection Transposases/genetics,metabolism
Chemicals
Transposases
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Zagoraiou L
Institute for Molecular Biology and Biotechnology-Foundation for Research and Technology, Hellas, P.O. Box 1527, Heraklion 71110, Greece.
Drabek D
Alexaki S
Guy J A
Klinakis A G
Langeveld A
Skavdis G
Mamalaki C
Grosveld F
Savakis C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.201392398
Published
2001-09-25
Epub
2001-00-18
Pages
11474-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC58754
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.201392398 PMC Full Text View journal details
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