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PMID: 2901953 Published · ppublish English Journal Article

The structure of the human CD2 gene and its expression in transgenic mice.

The EMBO journal ·Vol. 7 ·No. 6 ·1988-06-00 ·Pages 1675-82

Lang G, Wotton D, Owen MJ, Sewell WA, Brown MH, Mason DY, Crumpton MJ, Kioussis D

Abstract

We report the genomic organization of the human CD2 gene and its expression in transgenic mice. A 28.5 kb segment of DNA consisting of 4.5 kb 5' flanking sequences, 15 kb containing the gene's five exons and 9 kb of 3' flanking sequences can direct the expression of the CD2 gene only on thymocytes, circulating T cells and megakaryocytes of the transgenic mice. The expression of each copy of the human CD2 transgene appears to be as high as the endogenous mouse CD2 gene and as high as the expression on the surface of human T lymphocytes, independent of the site of integration and dependent on the copy number of genes that have integrated.

MeSH Terms
Amino Acid Sequence Animals Antigens, Differentiation, T-Lymphocyte/biosynthesis,genetics Base Sequence Bone Marrow/analysis CD2 Antigens Gene Expression Regulation Genes Humans Lymphoid Tissue/analysis Mice Mice, Transgenic Molecular Sequence Data RNA, Messenger/biosynthesis Receptors, Immunologic/biosynthesis,genetics Recombinant Proteins/biosynthesis,genetics Spleen/analysis
Chemicals
Antigens, Differentiation, T-Lymphocyte CD2 Antigens RNA, Messenger Receptors, Immunologic Recombinant Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Lang G
Laboratory of Gene Structure and Expression, National Institute for Medical Research, Mill Hill, London, UK.
Wotton D
Owen M J
Sewell W A
Brown M H
Mason D Y
Crumpton M J
Kioussis D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-06-00
Pages
1675-82
Language
English
Region
England
NLM ID
8208664
PMCID
PMC457152
Subset
IM
Databases
GENBANK
X07871, X07872, X07873, X07874
External Links
PubMed source PMC Full Text View journal details
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