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PMID: 15126400 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Quantitative trait loci affecting starvation resistance in Drosophila melanogaster.

Genetics ·Vol. 166 ·No. 4 ·2004-04-00 ·Pages 1807-23

Harbison ST, Yamamoto AH, Fanara JJ, Norga KK, Mackay TF

Abstract

The ability to withstand periods of scarce food resources is an important fitness trait. Starvation resistance is a quantitative trait controlled by multiple interacting genes and exhibits considerable genetic variation in natural populations. This genetic variation could be maintained in the face of strong selection due to a trade-off in resource allocation between reproductive activity and individual survival. Knowledge of the genes affecting starvation tolerance and the subset of genes that affect variation in starvation resistance in natural populations would enable us to evaluate this hypothesis from a quantitative genetic perspective. We screened 933 co-isogenic P-element insertion lines to identify candidate genes affecting starvation tolerance. A total of 383 P-element insertions induced highly significant and often sex-specific mutational variance in starvation resistance. We also used deficiency complementation mapping followed by complementation to mutations to identify 12 genes contributing to variation in starvation resistance between two wild-type strains. The genes we identified are involved in oogenesis, metabolism, and feeding behaviors, indicating a possible link to reproduction and survival. However, we also found genes with cell fate specification and cell proliferation phenotypes, which implies that resource allocation during development and at the cellular level may also influence the phenotypic response to starvation.

MeSH Terms
Analysis of Variance Animals Crosses, Genetic DNA Transposable Elements/genetics Drosophila melanogaster/genetics,physiology Genes, Insect/genetics,physiology Genetic Complementation Test Genetic Variation Mutation/genetics Quantitative Trait Loci Starvation/genetics
Chemicals
DNA Transposable Elements
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Harbison Susan T
Department of Genetics and W M Keck Center for Behavioral Biology, North Carolina State University, Raleigh, North Carolina 27695, USA.
Yamamoto Akihiko H
Fanara Juan J
Norga Koenraad K
Mackay Trudy F C
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.166.4.1807
Published
2004-04-00
Pages
1807-23
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1470806
Subset
IM
Grants
NHGRI NIH HHS · HG00750-08S1 · United States
NIGMS NIH HHS · GM59469 · United States
NIGMS NIH HHS · GM068949 · United States
NIGMS NIH HHS · GM45344 · United States
NIGMS NIH HHS · GM45146 · United States
External Links
PubMed source DOI: 10.1534/genetics.166.4.1807 PMC Full Text View journal details
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