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PMID: 11156986 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genetic mapping of quantitative trait loci governing longevity of Caenorhabditis elegans in recombinant-inbred progeny of a Bergerac-BO x RC301 interstrain cross.

Genetics ·Vol. 157 ·No. 2 ·2001-02-00 ·Pages 655-66

Ayyadevara S, Ayyadevara R, Hou S, Thaden JJ, Shmookler Reis RJ

Abstract

Recombinant-inbred populations, generated from a cross between Caenorhabditis elegans strains Bergerac-BO and RC301, were used to identify quantitative trait loci (QTL) affecting nematode longevity. Genotypes of young controls and longevity-selected worms (the last-surviving 1% from a synchronously aged population) were assessed at dimorphic transposon-specific markers by multiplex polymerase chain reaction. The power of genetic mapping was enhanced, in a novel experimental design, through map expansion by accrual of recombinations over several generations, internally controlled longevity selection from a genetically heterogeneous, homozygous population, and selective genotyping of extremely long-lived worms. Analysis of individual markers indicated seven life-span QTL, situated near markers on chromosomes I (tcbn2), III (stP127), IV (stP13), V (stP6, stP23, and stP128), and X (stP41). These loci were corroborated, and mapped with increased precision, by nonparametric interval mapping-which supported all loci implicated by single-marker analysis. In addition, a life-span QTL on chromosome II (stP100-stP196), was significant only by interval mapping. Congenic lines were constructed for the longevity QTL on chromosomes III and X, by backcrossing the Bergerac-BO QTL allele into an RC301 background with selection for flanking markers. Survival data for these lines demonstrated consistent and significant effects of each QTL on life span.

MeSH Terms
Alleles Animals Caenorhabditis elegans/genetics,physiology Chromosome Mapping Crosses, Genetic Epistasis, Genetic Genetic Markers Genotype Heterozygote Homozygote Polymorphism, Genetic Quantitative Trait, Heritable Recombination, Genetic Time Factors
Chemicals
Genetic Markers
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ayyadevara S
Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
Ayyadevara R
Hou S
Thaden J J
Shmookler Reis R J
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/157.2.655
Published
2001-02-00
Pages
655-66
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1461506
Subset
IM
Grants
NIA NIH HHS · R01-AG091413 · United States
External Links
PubMed source DOI: 10.1093/genetics/157.2.655 PMC Full Text View journal details
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