Home LiteratureArticle Details
PMID: 16968779 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

The transcriptome of human oocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 103 ·No. 38 ·2006-09-19 ·Pages 14027-32

Kocabas AM, Crosby J, Ross PJ, Otu HH, Beyhan Z, Can H, Tam WL, Rosa GJ, Halgren RG, Lim B, Fernandez E, Cibelli JB

Abstract

The identification of genes and deduced pathways from the mature human oocyte can help us better understand oogenesis, folliculogenesis, fertilization, and embryonic development. Human metaphase II oocytes were used within minutes after removal from the ovary, and its transcriptome was compared with a reference sample consisting of a mixture of total RNA from 10 different normal human tissues not including the ovary. RNA amplification was performed by using a unique protocol. Affymetrix Human Genome U133 Plus 2.0 GeneChip arrays were used for hybridizations. Compared with reference samples, there were 5,331 transcripts significantly up-regulated and 7,074 transcripts significantly down-regulated in the oocyte. Of the oocyte up-regulated probe sets, 1,430 have unknown function. A core group of 66 transcripts was identified by intersecting significantly up-regulated genes of the human oocyte with those from the mouse oocyte and from human and mouse embryonic stem cells. GeneChip array results were validated using RT-PCR in a selected set of oocyte-specific genes. Within the up-regulated probe sets, the top overrepresented categories were related to RNA and protein metabolism, followed by DNA metabolism and chromatin modification. This report provides a comprehensive expression baseline of genes expressed in in vivo matured human oocytes. Further understanding of the biological role of these genes may expand our knowledge on meiotic cell cycle, fertilization, chromatin remodeling, lineage commitment, pluripotency, tissue regeneration, and morphogenesis.

MeSH Terms
Animals Female Gene Expression Profiling Gene Expression Regulation Humans Mice Oligonucleotide Array Sequence Analysis Oocytes/physiology Reproducibility of Results Transcription, Genetic
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Kocabas Arif Murat
Cellular Reprogramming Laboratory, Department of Animal Science, Michigan State University, East Lansing, MI 48824, USA.
Crosby Javier
Ross Pablo J
Otu Hasan H
Beyhan Zeki
Can Handan
Tam Wai-Leong
Rosa Guilherme J M
Halgren Robert G
Lim Bing
Fernandez Emilio
Cibelli Jose Bernardo
References (48)
48 references, click to expand
  1. Transcription of homeobox-containing genes detected in cDNA libraries derived from human unfertilized oocytes and preimplantation embryos.
    Mol Hum Reprod. 2000 Aug;6(8):707-11 PMID: 10908280
  2. Transcript profiling during preimplantation mouse development.
    Dev Biol. 2004 Aug 15;272(2):483-96 PMID: 15282163
  3. Human gene expression first occurs between the four- and eight-cell stages of preimplantation development.
    Nature. 1988 Mar 31;332(6163):459-61 PMID: 3352746
  4. Gene expression during oogenesis and oocyte development in mammals.
    Dev Biol (N Y 1985). 1985;1:453-524 PMID: 2481471
  5. Gene expression during oogenesis in mice.
    Mutat Res. 1992 Dec;296(1-2):3-15 PMID: 1279405
  6. Homeobox gene expression in human oocytes and preembryos.
    Mol Reprod Dev. 1995 Jun;41(2):127-32 PMID: 7654365
  7. Simultaneous detection of multiple gene expression in mouse and human individual preimplantation embryos.
    Fertil Steril. 1997 Apr;67(4):733-41 PMID: 9093203
  8. Transcription of tissue-specific genes in human preimplantation embryos.
    Hum Reprod. 1997 Oct;12(10):2251-6 PMID: 9402290
  9. The construction of cDNA libraries from human single preimplantation embryos and their use in the study of gene expression during development.
    J Assist Reprod Genet. 1998 May;15(5):344-8 PMID: 9604772
  10. Detection of a novel splice variant of the hypoxanthine-guanine phosphoribosyl transferase gene in human oocytes and preimplantation embryos: implications for a RT-PCR-based preimplantation diagnosis of Lesch-Nyhan syndrome.
    Mol Hum Reprod. 1998 Aug;4(8):785-9 PMID: 9733436
  11. Differential expression of estrogen receptor-beta and estrogen receptor-alpha in the rat ovary.
    Endocrinology. 1999 Feb;140(2):963-71 PMID: 9927330
  12. Stage-specific expression of estrogen receptor subtypes and estrogen responsive finger protein in preimplantational mouse embryos.
    Endocr J. 1999 Feb;46(1):153-8 PMID: 10426580
  13. Identification of genes expressed in human primordial germ cells at the time of entry of the female germ line into meiosis.
    Mol Hum Reprod. 1999 Sep;5(9):851-60 PMID: 10460224
  14. Developmental expression of specific genes detected in high-quality cDNA libraries from single human preimplantation embryos.
    Gene. 1999 Sep 17;237(2):373-83 PMID: 10521661
  15. Comparison of seven methods for producing Affymetrix expression scores based on False Discovery Rates in disease profiling data.
    BMC Bioinformatics. 2005;6:26 PMID: 15705192
  16. Whole-genome approaches for large-scale gene identification and expression analysis in mammalian preimplantation embryos.
    Reprod Fertil Dev. 2005;17(1-2):37-45 PMID: 15745630
  17. DAZL expression in human oocytes, preimplantation embryos and embryonic stem cells.
    Mol Hum Reprod. 2005 Jun;11(6):405-11 PMID: 15879466
  18. Nuclear reprogramming of somatic cells after fusion with human embryonic stem cells.
    Science. 2005 Aug 26;309(5739):1369-73 PMID: 16123299
  19. Transcript profiling during mouse oocyte development and the effect of gonadotropin priming and development in vitro.
    Dev Biol. 2005 Oct 15;286(2):493-506 PMID: 16168984
  20. Expression of Polycomb-group genes in human ovarian follicles, oocytes and preimplantation embryos.
    Reproduction. 2005 Dec;130(6):883-8 PMID: 16322547
  21. Analysis of gene expression in single human oocytes and preimplantation embryos.
    Biochem Biophys Res Commun. 2006 Feb 3;340(1):48-53 PMID: 16343430
  22. The use of amplified cDNA to investigate the expression of seven imprinted genes in human oocytes and preimplantation embryos.
    Mol Hum Reprod. 2001 Sep;7(9):839-44 PMID: 11517290
  23. Isolation of novel developmental genes from human germ cell, oocyte and embryo cDNA by differential display.
    Reprod Fertil Dev. 2001;13(1):51-7 PMID: 11545165
  24. Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells.
    Curr Biol. 2001 Oct 2;11(19):1553-8 PMID: 11591326
  25. Embryogenomics: developmental biology meets genomics.
    Trends Biotechnol. 2001 Dec;19(12):511-8 PMID: 11711195
  26. Cloned cattle can be healthy and normal.
    Science. 2001 Nov 30;294(5548):1893-4 PMID: 11729307
  27. Cloning of HOXD1 from unfertilised human oocytes and expression analyses during murine oogenesis and embryogenesis.
    Mech Dev. 2001 Dec;109(2):377-81 PMID: 11731253
  28. Identification and characterisation of known and novel transcripts expressed during the final stages of human oocyte maturation.
    Mol Reprod Dev. 2002 May;62(1):13-28 PMID: 11933157
  29. Application of cDNA arrays to monitor mRNA profiles in single preimplantation mouse embryos.
    Biotechniques. 2002 Aug;33(2):376-8, 380, 382-5 PMID: 12188190
  30. Isolation, characterization and expression of the human Factor In the Germline alpha (FIGLA) gene in ovarian follicles and oocytes.
    Mol Hum Reprod. 2002 Dec;8(12):1087-95 PMID: 12468641
  31. The human cumulus--oocyte complex gene-expression profile.
    Hum Reprod. 2006 Jul;21(7):1705-19 PMID: 16571642
  32. Summaries of Affymetrix GeneChip probe level data.
    Nucleic Acids Res. 2003 Feb 15;31(4):e15 PMID: 12582260
  33. Derivation of oocytes from mouse embryonic stem cells.
    Science. 2003 May 23;300(5623):1251-6 PMID: 12730498
  34. Nuclei of adult mammalian somatic cells are directly reprogrammed to oct-4 stem cell gene expression by amphibian oocytes.
    Curr Biol. 2003 Jul 15;13(14):1206-13 PMID: 12867031
  35. Molecular signature of human embryonic stem cells and its comparison with the mouse.
    Dev Biol. 2003 Aug 15;260(2):404-13 PMID: 12921741
  36. Identifying biological themes within lists of genes with EASE.
    Genome Biol. 2003;4(10):R70 PMID: 14519205
  37. Comment on " 'Stemness': transcriptional profiling of embryonic and adult stem cells" and "a stem cell molecular signature".
    Science. 2003 Oct 17;302(5644):393; author reply 393 PMID: 14563990
  38. Derivation of embryonic germ cells and male gametes from embryonic stem cells.
    Nature. 2004 Jan 8;427(6970):148-54 PMID: 14668819
  39. Dynamics of global gene expression changes during mouse preimplantation development.
    Dev Cell. 2004 Jan;6(1):117-31 PMID: 14723852
  40. A genome-wide study of gene activity reveals developmental signaling pathways in the preimplantation mouse embryo.
    Dev Cell. 2004 Jan;6(1):133-44 PMID: 14723853
  41. Expression of mRNAs for DNA methyltransferases and methyl-CpG-binding proteins in the human female germ line, preimplantation embryos, and embryonic stem cells.
    Mol Reprod Dev. 2004 Mar;67(3):323-36 PMID: 14735494
  42. Decreased expression of mitochondrial genes in human unfertilized oocytes and arrested embryos.
    Fertil Steril. 2004 Mar;81 Suppl 1:912-8 PMID: 15019829
  43. Expression profiles of individual human oocytes using microarray technology.
    Reprod Biomed Online. 2004 Mar;8(3):325-37 PMID: 15038899
  44. Estrogen is not directly required for oocyte developmental competence.
    Biol Reprod. 2004 May;70(5):1263-9 PMID: 14695903
  45. A gene atlas of the mouse and human protein-encoding transcriptomes.
    Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):6062-7 PMID: 15075390
  46. A global view of gene expression in the preimplantation mouse embryo: morula versus blastocyst.
    Eur J Obstet Gynecol Reprod Biol. 2004 Jul 1;115 Suppl 1:S85-91 PMID: 15196723
  47. The unique transcriptome through day 3 of human preimplantation development.
    Hum Mol Genet. 2004 Jul 15;13(14):1461-70 PMID: 15150160
  48. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0603227103
Published
2006-09-19
Epub
2006-00-12
Pages
14027-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1599906
Subset
IM
Grants
NIDDK NIH HHS · R01 DK047636 · United States
NIDDK NIH HHS · DK047636 · United States
External Links
PubMed source DOI: 10.1073/pnas.0603227103 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2