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PMID: 17005721 Published · ppublish English Comparative Study Journal Article

piggyBac is a flexible and highly active transposon as compared to sleeping beauty, Tol2, and Mos1 in mammalian cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 103 ·No. 41 ·2006-10-10 ·Pages 15008-13

Wu SC, Meir YJ, Coates CJ, Handler AM, Pelczar P, Moisyadi S, Kaminski JM

Abstract

A nonviral vector for highly efficient site-specific integration would be desirable for many applications in transgenesis, including gene therapy. In this study we directly compared the genomic integration efficiencies of piggyBac, hyperactive Sleeping Beauty (SB11), Tol2, and Mos1 in four mammalian cell lines. piggyBac demonstrated significantly higher transposition activity in all cell lines whereas Mos1 had no activity. Furthermore, piggyBac transposase coupled to the GAL4 DNA-binding domain retains transposition activity whereas similarly manipulated gene products of Tol2 and SB11 were inactive. The high transposition activity of piggyBac and the flexibility for molecular modification of its transposase suggest the possibility of using it routinely for mammalian transgenesis.

MeSH Terms
Animals CHO Cells Cell Line Cell Line, Tumor Cricetinae Cricetulus DNA Transposable Elements/genetics,physiology DNA-Binding Proteins/chemistry,genetics,physiology HeLa Cells Humans Insect Proteins/genetics,physiology Moths/virology Mutagenesis, Insertional Mutagenesis, Site-Directed Transposases/chemistry,genetics,physiology
Chemicals
DNA Transposable Elements DNA-Binding Proteins Insect Proteins mariner transposases Transposases sleeping beauty transposase, human
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wu Sareina Chiung-Yuan
Cancer Research Center, Medical College of Georgia, Augusta, GA 30912, USA.
Meir Yaa-Jyuhn James
Coates Craig J
Handler Alfred M
Pelczar Pawel
Moisyadi Stefan
Kaminski Joseph M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0606979103
Published
2006-10-10
Epub
2006-00-27
Pages
15008-13
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1622771
Subset
IM
Corrections
CommentIn
PMID: 17015820
External Links
PubMed source DOI: 10.1073/pnas.0606979103 PMC Full Text View journal details
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