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PMID: 1701020 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of a transcription factor, AGP/EBP, that belongs to members of the C/EBP family.

Molecular and cellular biology ·Vol. 10 ·No. 12 ·1990-12-00 ·Pages 6642-53

Chang CJ, Chen TT, Lei HY, Chen DS, Lee SC

Abstract

A C/EBP-like transcription factor, AGP/EBP, that binds to three distinct motifs in the 5'-flanking region of alpha 1-acid glycoprotein gene (AGP) has been identified. Here we report the cloning and properties of cDNA corresponding to mouse AGP/EBP. AGP/EBP and C/EBP share 87% amino acid sequence homology in the "leucine zipper" and its associated DNA-binding domains, while their sequences outside these domains and the sizes of their mRNAs are different. Unlike the limited expression of C/EBP in tissues and cells, AGP/EBP appears to be ubiquitously expressed in tissues like lung, spleen, kidney, heart, testis, and liver and cell lines like p388D1, 129P (hepatoma cell line of C3H/HeJ), FO (mouse myeloma), and L929. Antibody against cloned and expressed AGP/EBP which was raised in rabbits could recognize AGP/EBP from nuclear extract of a number of cells and tissues. On the basis of our findings about the structural relationship and the similarity of motif recognition, we propose that a family of C/EBP-like transcription factors exists.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern CCAAT-Enhancer-Binding Proteins Cloning, Molecular DNA-Binding Proteins/genetics Gene Library Immunoblotting Liver/metabolism Mice Molecular Sequence Data Multigene Family Nuclear Proteins/genetics Nucleotide Mapping Oligonucleotide Probes Poly A/genetics Protein Biosynthesis RNA/genetics,isolation & purification RNA, Messenger Sequence Homology, Nucleic Acid Transcription Factors/genetics Transcription, Genetic
Chemicals
CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins Nuclear Proteins Oligonucleotide Probes RNA, Messenger Transcription Factors Poly A RNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chang C J
Institute of Biological Chemistry, Academia Sinica, Taiwan.
Chen T T
Lei H Y
Chen D S
Lee S C
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.12.6642-6653.1990
Published
1990-12-00
Pages
6642-53
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362942
Subset
IM
Databases
GENBANK
M61007
External Links
PubMed source DOI: 10.1128/mcb.10.12.6642-6653.1990 PMC Full Text View journal details
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