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PMID: 17060497 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

MyoD-positive epiblast cells regulate skeletal muscle differentiation in the embryo.

The Journal of cell biology ·Vol. 175 ·No. 2 ·2006-10-23 ·Pages 283-92

Gerhart J, Elder J, Neely C, Schure J, Kvist T, Knudsen K, George-Weinstein M

Abstract

MyoD mRNA is expressed in a subpopulation of cells within the embryonic epiblast. Most of these cells are incorporated into somites and synthesize Noggin. Ablation of MyoD-positive cells in the epiblast subsequently results in the herniation of organs through the ventral body wall, a decrease in the expression of Noggin, MyoD, Myf5, and myosin in the somites and limbs, and an increase in Pax-3-positive myogenic precursors. The addition of Noggin lateral to the somites compensates for the loss of MyoD-positive epiblast cells. Skeletal muscle stem cells that arise in the epiblast are utilized in the somites to promote muscle differentiation by serving as a source of Noggin.

MeSH Terms
Animals Carrier Proteins/metabolism Cell Differentiation/physiology Chick Embryo Embryo, Mammalian/cytology,physiology Embryo, Nonmammalian Epithelium/anatomy & histology,physiology Extremities Fluorescent Antibody Technique Gene Expression Regulation, Developmental In Situ Hybridization Morphogenesis Muscle, Skeletal/cytology,embryology,metabolism MyoD Protein/genetics,physiology Myogenic Regulatory Factor 5/metabolism Myosins/metabolism Paired Box Transcription Factors/metabolism Somites/metabolism Stem Cells/chemistry,cytology
Chemicals
Carrier Proteins MyoD Protein Myogenic Regulatory Factor 5 Paired Box Transcription Factors noggin protein Myosins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Gerhart Jacquelyn
Center for Chronic Disorders of Aging, Philadelphia College of Osteopathic Medicine, Philadelphia, PA 19131, USA.
Elder Justin
Neely Christine
Schure Jared
Kvist Tage
Knudsen Karen
George-Weinstein Mindy
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200605037
Published
2006-10-23
Pages
283-92
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2064569
Subset
IM
Grants
NIGMS NIH HHS · R01 GM061702 · United States
NIAMS NIH HHS · AR052326 · United States
NIAMS NIH HHS · R01 AR052326 · United States
NIGMS NIH HHS · GM61702 · United States
NICHD NIH HHS · R01 HD043157 · United States
NICHD NIH HHS · HD43157 · United States
External Links
PubMed source DOI: 10.1083/jcb.200605037 PMC Full Text View journal details
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