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PMID: 1706322 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mapping of B-cell epitopes on the outer membrane P2 porin protein of Haemophilus influenzae by using recombinant proteins and synthetic peptides.

Infection and immunity ·Vol. 59 ·No. 4 ·1991-04-00 ·Pages 1457-64

Martin D, Munson R, Grass S, Chong P, Hamel J, Zobrist G, Klein M, Brodeur BR

Abstract

The P2 protein of Haemophilus influenzae type b has a porin activity and is the most abundant protein in the outer membrane. We have employed fusion protein constructs and synthetic peptides along with monoclonal antibodies to map B-cell epitopes in this protein. A linear, surface-exposed epitope was identified between residues 158 and 174. A second surface-exposed epitope was identified near the carboxy-terminal end of the protein (residues 319 to 341). Two additional B-cell epitopes were identified. One was localized between residues 28 and 55, whereas the other was located between residues 148 and 174. These epitopes were not present on the surface of intact H. influenzae cells. Thus, four distinct immunogenic and antigenic regions on the P2 protein have been identified.

MeSH Terms
Amino Acid Sequence Antibodies, Monoclonal Bacterial Outer Membrane Proteins/immunology Base Sequence Enzyme-Linked Immunosorbent Assay Epitopes/analysis Haemophilus influenzae/immunology Molecular Sequence Data Peptide Fragments/immunology Porins Recombinant Proteins/immunology
Chemicals
Antibodies, Monoclonal Bacterial Outer Membrane Proteins Epitopes Peptide Fragments Porins Recombinant Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Martin D
National Laboratory for Immunology, Laboratory Centre for Disease Control, Ottawa, Canada.
Munson R
Grass S
Chong P
Hamel J
Zobrist G
Klein M
Brodeur B R
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.59.4.1457-1464.1991
Published
1991-04-00
Pages
1457-64
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC257863
Subset
IM
Grants
NIAID NIH HHS · R01 AI 17572 · United States
External Links
PubMed source DOI: 10.1128/iai.59.4.1457-1464.1991 PMC Full Text View journal details
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