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PMID: 3263324 Published · ppublish English Journal Article

Nearest neighbor analysis of outer membrane proteins of nontypeable Haemophilus influenzae.

Infection and immunity ·Vol. 56 ·No. 12 ·1988-12-00 ·Pages 3058-63

Klingman KL, Jansen EM, Murphy TF

Abstract

The arrangement of outer membrane proteins on the surface of nontypeable Haemophilus influenzae was investigated with cleavable and noncleavable bis-imidate cross-linking agents. Whole organisms were subjected to cross-linking agents, and oligomers of proteins were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, two-dimensional gel electrophoresis, and immunoblot assay, using monoclonal antibodies to outer membrane proteins. The major outer membrane protein (P2) formed dimers and trimers detected by all three methods. Oligomers of other outer membrane proteins were not detected. These data indicate that P2 exists as a trimer on the outer membrane and suggest that other outer membrane proteins exist as monomers on the outer membrane.

MeSH Terms
Antibodies, Monoclonal/immunology Bacterial Outer Membrane Proteins/analysis,immunology Cross-Linking Reagents Electrophoresis, Gel, Two-Dimensional Haemophilus influenzae/immunology,ultrastructure Imides Immunoblotting Macromolecular Substances Molecular Weight
Chemicals
Antibodies, Monoclonal Bacterial Outer Membrane Proteins Cross-Linking Reagents Imides Macromolecular Substances
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Klingman K L
Department of Medicine, State University of New York, Buffalo 14215.
Jansen E M
Murphy T F
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.56.12.3058-3063.1988
Published
1988-12-00
Pages
3058-63
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259700
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.56.12.3058-3063.1988 PMC Full Text View journal details
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