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PMID: 3491049 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Antigenic characterization of the P6 protein of nontypable Haemophilus influenzae.

Infection and immunity ·Vol. 54 ·No. 3 ·1986-12-00 ·Pages 774-9

Murphy TF, Bartos LC, Campagnari AA, Nelson MB, Apicella MA

Abstract

The purpose of this study was to characterize the degree of antigenic heterogeneity or conservation of a 16,600-dalton outer membrane protein (P6) among strains of nontypable Haemophilus influenzae. Immunization of rabbits with P6 isolated from individual strains resulted in antibody to P6 of all 25 strains tested. The titers of antibody in the sera were similar among the strains. Whole organisms of two strains were used to immunize rabbits, and antibodies were produced to P6 of all strains tested. Monoclonal antibodies developed to P6 from mice immunized with whole cells of three different strains recognized determinants on P6 of all 25 strains tested. Finally, pooled normal human serum contained antibodies to P6 of all 25 strains assayed. These studies indicate that P6 is a highly conserved antigen on the outer membrane of nontypable H. influenzae.

MeSH Terms
Animals Antibodies, Bacterial/immunology Antibodies, Monoclonal/immunology Antigens, Bacterial/immunology Bacterial Outer Membrane Proteins/immunology Cross Reactions Haemophilus influenzae/classification,immunology Humans Rabbits Species Specificity
Chemicals
Antibodies, Bacterial Antibodies, Monoclonal Antigens, Bacterial Bacterial Outer Membrane Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Murphy T F
Bartos L C
Campagnari A A
Nelson M B
Apicella M A
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18 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.54.3.774-779.1986
Published
1986-12-00
Pages
774-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260236
Subset
IM
Grants
NIAID NIH HHS · AI19641 · United States
External Links
PubMed source DOI: 10.1128/iai.54.3.774-779.1986 PMC Full Text View journal details
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