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PMID: 17101772 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Normal lymphatic development and function in mice deficient for the lymphatic hyaluronan receptor LYVE-1.

Molecular and cellular biology ·Vol. 27 ·No. 2 ·2007-01-00 ·Pages 595-604

Gale NW, Prevo R, Espinosa J, Ferguson DJ, Dominguez MG, Yancopoulos GD, Thurston G, Jackson DG

Abstract

The hyaluronan receptor LYVE-1 is expressed abundantly on the surfaces of lymphatic vessels and lymph node sinus endothelial cells from early development, where it has been suggested to function both in cell adhesion/transmigration and as a scavenger for hyaluronan turnover. To investigate the physiological role(s) of LYVE-1, we generated mice in which the gene for the receptor was inactivated by replacement with a beta-galactosidase reporter. LYVE-1(-/-) mice displayed an apparently normal phenotype, with no obvious alteration in lymphatic vessel ultrastructure or function and no apparent change in secondary lymphoid tissue structure or cellularity. In addition, the levels of hyaluronan in tissue and blood were unchanged. LYVE-1(-/-) mice also displayed normal trafficking of cutaneous CD11c(+) dendritic cells to draining lymph nodes via afferent lymphatics and normal resolution of oxazolone-induced skin inflammation. Finally, LYVE-1(-/-) mice supported normal growth of transplanted B16F10 melanomas and Lewis lung carcinomas. These results indicate that LYVE-1 is not obligatory for normal lymphatic development and function and suggest either the existence of compensatory receptors or a role more specific than that previously envisaged.

MeSH Terms
Animals CD11c Antigen/metabolism Carcinoma, Lewis Lung/pathology Cell Movement Dendritic Cells/physiology Dermatitis, Contact/etiology,immunology Glycoproteins/genetics,physiology Hyaluronic Acid/blood,metabolism Inflammation/chemically induced,immunology Lymph Nodes/cytology,metabolism,physiology Lymphatic Vessels/cytology,metabolism,physiology Melanoma/pathology Membrane Transport Proteins Mice Mice, Knockout Neoplasm Transplantation Oxazolone beta-Galactosidase/genetics
Chemicals
CD11c Antigen Glycoproteins Membrane Transport Proteins Xlkd1 protein, mouse Oxazolone Hyaluronic Acid beta-Galactosidase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Gale Nicholas W
MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, United Kingdom.
Prevo Remko
Espinosa Jorge
Ferguson David J
Dominguez Melissa G
Yancopoulos George D
Thurston Gavin
Jackson David G
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.01503-06
Published
2007-01-00
Epub
2006-00-13
Pages
595-604
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1800809
Subset
IM
Grants
Medical Research Council · MC_U137884182 · United Kingdom
External Links
PubMed source DOI: 10.1128/MCB.01503-06 PMC Full Text View journal details
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