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PMID: 1713034 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Lack of 5-methylcytosine in Dictyostelium discoideum DNA.

The Biochemical journal ·Vol. 277 ( Pt 1) ·1991-07-01 ·Pages 273-5

Smith SS, Ratner DI

Abstract

We find no evidence for the presence of 5-methylcytosine in the DNA of Dictyostelium discoideum. Methylation was absent from CCGG sites in repetitive DNA and in DNA from the actin multigene family. Nor was 5-methylcytosine detected in total DNA when base composition was determined by means of h.p.l.c.

MeSH Terms

5-Methylcytosine Actins/genetics Base Sequence Cytosine/analogs & derivatives,analysis DNA, Fungal/chemistry,genetics,isolation & purification Deoxyribonuclease HpaII Deoxyribonucleases, Type II Site-Specific Dictyostelium/genetics Genes, Fungal Restriction Mapping

Chemicals

Actins DNA, Fungal 5-Methylcytosine Cytosine Deoxyribonuclease HpaII Deoxyribonucleases, Type II Site-Specific

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Smith S S

Department of Cell and Tumor Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010.

Ratner D I

References (15)

15 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2770273

Published

1991-07-01

Pages

273-5

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1151219

Subset

Grants

NIGMS NIH HHS · GM38350-02 · United States

External Links

PubMed source DOI: 10.1042/bj2770273 PMC Full Text View journal details

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Lack of 5-methylcytosine in Dictyostelium discoideum DNA.

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