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PMID: 17170049 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Blood and synovial fluid cytokine signatures in patients with juvenile idiopathic arthritis: a cross-sectional study.

Annals of the rheumatic diseases ·Vol. 66 ·No. 5 ·2007-05-00 ·Pages 589-98

de Jager W, Hoppenreijs EP, Wulffraat NM, Wedderburn LR, Kuis W, Prakken BJ

Abstract

Juvenile idiopathic arthritis (JIA) consists of a heterogeneous group of disorders with, for the most part, an unknown immunopathogenesis. Although onset and disease course differ, the subtypes of JIA share the occurrence of chronic inflammation of the joints, with infiltrations of immunocompetent cells that secrete inflammatory mediators. To identify a panel of cytokines specifically related to the inflammatory process in JIA. Using a new technology, the multiplex immunoassay, 30 cytokines were measured in plasma of 65 patients with JIA, of which 34 were paired with synovial fluid. These data were compared with plasma of 20 healthy controls and 9 patients with type I diabetes, a chronic inflammatory disease. Patients with JIA had, irrespective of their subclassification, significantly higher levels of tumour necrosis factor alpha, macrophage inhibitory factor (MIF), CCL2, CCL3, CCL11, CCL22 and CXCL9 in plasma than controls. In paired plasma and synovial fluid samples of patients with JIA, significantly higher levels of interleukin (IL)6, IL15, CCL2, CCL3, CXCL8, CXCL9 and CXCL10 were present in synovial fluid. Cluster analysis in all patients with JIA revealed a predominant pro-inflammatory cytokine cluster during active disease and a regulatory/anti-inflammatory-related cytokine cluster during remission. Whether a discrimination profile of various cytokines could help in the determination of disease classification was tested. It is suggested that several cytokines (IL18, MIF, CCL2, CCL3, CCL11, CXCL9 and CXCL10) may correspond to the activation status during inflammation in JIA and could be instrumental in monitoring disease activity and outcomes of (new) immunotherapies.

MeSH Terms
Adolescent Arthritis, Juvenile/blood,immunology Chemokines/analysis,blood Child Child, Preschool Cross-Sectional Studies Cytokines/analysis,blood Diabetes Mellitus, Type 1/blood,immunology Discriminant Analysis Female Humans Immunoassay/methods Interleukins/analysis,blood Macrophage Migration-Inhibitory Factors/analysis,blood Male Synovial Fluid/immunology Tumor Necrosis Factor-alpha/analysis,blood
Chemicals
Chemokines Cytokines Interleukins Macrophage Migration-Inhibitory Factors Tumor Necrosis Factor-alpha
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
de Jager Wilco
Department of Pediatric Immunology, University Medical Center Utrecht, Utrecht, The Netherlands.
Hoppenreijs Esther P A H
Wulffraat Nico M
Wedderburn Lucy R
Kuis Wietse
Prakken Berent J
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Article Info
Journal
Annals of the rheumatic diseases
Abbr.
Ann Rheum Dis
ISSN
0003-4967
DOI
10.1136/ard.2006.061853
Published
2007-05-00
Epub
2006-00-14
Pages
589-98
Language
English
Region
England
NLM ID
0372355
PMCID
PMC1954617
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1136/ard.2006.061853 PMC Full Text View journal details
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