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PMID: 17171797 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Blockade of high mobility group box-1 protein attenuates experimental severe acute pancreatitis.

World journal of gastroenterology ·Vol. 12 ·No. 47 ·2006-12-21 ·Pages 7666-70

Sawa H, Ueda T, Takeyama Y, Yasuda T, Shinzeki M, Nakajima T, Kuroda Y

Abstract

To examine the effects of anti-high mobility group box 1 (HMGB1) neutralizing antibody in experimental severe acute pancreatitis (SAP). SAP was induced by creating closed duodenal loop in C3H/HeN mice. SAP was induced immediately after intraperitoneal injection of anti-HMGB1 neutralizing antibody (200 microg). Severity of pancreatitis, organ injury (liver, kidney and lung), and bacterial translocation to pancreas was examined 12 h after induction of SAP. Anti-HMGB1 neutralizing antibody significantly improved the elevation of the serum amylase level and the histological alterations of pancreas and lung in SAP. Anti-HMGB1 antibody also significantly ameliorated the elevations of serum alanine aminotransferase and creatinine in SAP. However, anti-HMGB1 antibody worsened the bacterial translocation to pancreas. Blockade of HMGB1 attenuated the development of SAP and associated organ dysfunction, suggesting that HMGB1 may act as a key mediator for inflammatory response and organ injury in SAP.

MeSH Terms
Animals Antibodies/pharmacology Female HMGB1 Protein/antagonists & inhibitors,immunology Immunotherapy/methods Mice Mice, Inbred C3H Pancreatitis/immunology,pathology,therapy Severity of Illness Index
Chemicals
Antibodies HMGB1 Protein
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Sawa Hidehiro
Department of Gastroenterological Surgery, Kobe University Graduate School of Medical Sciences, Kobe 650-0017, Japan. [email protected]
Ueda Takashi
Takeyama Yoshifumi
Yasuda Takeo
Shinzeki Makoto
Nakajima Takahiro
Kuroda Yoshikazu
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Article Info
Journal
World journal of gastroenterology
Abbr.
World J Gastroenterol
ISSN
1007-9327
DOI
10.3748/wjg.v12.i47.7666
Email
[email protected]
Published
2006-12-21
Pages
7666-70
Language
English
Region
United States
NLM ID
100883448
PMCID
PMC4088050
Subset
IM
External Links
PubMed source DOI: 10.3748/wjg.v12.i47.7666 PMC Full Text View journal details
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