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PMID: 1718521 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of transepithelial ion transport and intracellular calcium by extracellular ATP in human normal and cystic fibrosis airway epithelium.

British journal of pharmacology ·Vol. 103 ·No. 3 ·1991-07-00 ·Pages 1649-56

Mason SJ, Paradiso AM, Boucher RC

Abstract

1 The role of extracellular nucleotides in regulation of ion transport activities (short circuit current, Isc) of human respiratory epithelia was studied. 2 Application of nucleotides to the apical or basolateral membrane of human nasal epithelium induced a concentration-dependent increase in Isc. 3 The rank order of potency of purine- or pyrimidine-induced changes in Isc of normal human nasal epithelium when applied to the apical membrane (UTP greater than or equal to ATP greater than ATP gamma S greater than 2MeSATP greater than ADP beta S much greater than beta gamma MeATP greater than or equal to alpha beta MeATP) or basolateral membrane (2MeSATP greater than UTP greater than ATP greater than ATP gamma S greater than alpha beta MeATP greater than beta gamma MeATP) is consistent with involvement of a P2 purinoceptor. A similar rank order of potencies was observed for nucleotide effects on intracellular calcium measured by Fura-2 fluorescence using microspectrofluorimetry. 4 Similar nucleotide potency in the regulation of ion transport and intracellular calcium in cystic fibrosis (CF) airway epithelium (UTP greater than or equal to ATP) was observed, suggesting purinoceptors might be used to stimulate ion transport processes that would promote hydration of airway secretions and facilitate their clearance from CF lungs. 5 These data provide evidence for the regulation of ion transport by P2 purinoceptors in normal and cystic fibrosis human airway epithelium.

MeSH Terms
Adenosine Triphosphate/physiology Calcium/metabolism Chlorides/metabolism Cystic Fibrosis/metabolism Electrophysiology Epithelium/metabolism,physiology Humans In Vitro Techniques Ion Channels/metabolism,physiology Nasal Mucosa/cytology,drug effects,metabolism Nucleotides/pharmacology Receptors, Purinergic/drug effects,metabolism
Chemicals
Chlorides Ion Channels Nucleotides Receptors, Purinergic Adenosine Triphosphate Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mason S J
Department of Medicine, University of North Carolina Chapel Hill 27599.
Paradiso A M
Boucher R C
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41 references, click to expand
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Article Info
Journal
British journal of pharmacology
Abbr.
Br J Pharmacol
ISSN
0007-1188
DOI
10.1111/j.1476-5381.1991.tb09842.x
Published
1991-07-00
Pages
1649-56
Language
English
Region
England
NLM ID
7502536
PMCID
PMC1907816
Subset
IM
Grants
NHLBI NIH HHS · HL34322 · United States
NHLBI NIH HHS · HL42384 · United States
External Links
PubMed source DOI: 10.1111/j.1476-5381.1991.tb09842.x PMC Full Text View journal details
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