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PMID: 17194792 Published · ppublish English Journal Article

Colony spreading in Staphylococcus aureus.

Journal of bacteriology ·Vol. 189 ·No. 6 ·2007-03-00 ·Pages 2553-7

Kaito C, Sekimizu K

Abstract

Wild-type Staphylococcus aureus rapidly expands on the surface of soft agar plates. The rates of expansion and the shapes of the resultant giant colonies were distinct for different strains of laboratory stocks and clinical isolates. The colony spreading abilities did not correlate with the biofilm-forming abilities in these strains. Insertional disruption of the dltABCD operon, which functions at the step of D-alanine addition to teichoic acids, and of the tagO gene, which is responsible for the synthesis of wall teichoic acids, decreased the colony spreading ability. The results indicate that wall teichoic acids and D-alanylation of teichoic acids are required for colony spreading.

MeSH Terms
Agar Bacterial Proteins/genetics,metabolism Carrier Proteins/genetics,metabolism Culture Media Humans Membrane Transport Proteins/genetics,metabolism Movement Mutation Operon Staphylococcus aureus/genetics,growth & development,metabolism,physiology Teichoic Acids/metabolism Thiolester Hydrolases/genetics,metabolism
Chemicals
Bacterial Proteins Carrier Proteins Culture Media D-alanyl carrier protein, bacteria DltB protein, Staphylococcus Membrane Transport Proteins Teichoic Acids Agar DltD protein, bacteria Thiolester Hydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kaito Chikara
Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 3-1, 7-Chome, Hongo, Tokyo 113-0033, Japan.
Sekimizu Kazuhisa
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.01635-06
Published
2007-03-00
Epub
2006-00-28
Pages
2553-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1899384
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.01635-06 PMC Full Text View journal details
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