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PMID: 1720547 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Detection of homeobox genes in development and evolution.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 23 ·1991-12-01 ·Pages 10711-5

Murtha MT, Leckman JF, Ruddle FH

Abstract

The homeobox genes encode a family of DNA-binding regulatory proteins whose function and genomic organization make them an important model system for the study of development and differentiation. Oligonucleotide primers corresponding to highly conserved regions of Antennapediaclass homeodomains were designed to detect and identify homeobox sequences in populations of DNA or RNA by means of the polymerase chain reaction (PCR). Here we present a survey of sequences detected by PCR using an initial set of primers (HoxA and HoxB) based on an early nucleotide consensus for vertebrate Antennapedia-class homeodomains. Several novel sequences are reported from both mouse genomic DNA and RNA from the developing mouse telencephalon. Forebrain-derived clones are similar to the chicken CHox7, Drosophila H2.0, and mouse Hlx genes. PCR also proved to be a rapid method for identifying homeobox sequences from diverse metazoan species. Cloning of three Antennapedia-related sequences from cnidarians provides evidence of ancient roles for homeobox genes early in metazoan evolution.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Biological Evolution Ciona intestinalis/genetics Codon Diptera/genetics Drosophila/genetics Embryo, Mammalian Embryo, Nonmammalian Genes, Homeobox Male Mice Molecular Sequence Data Oligodeoxyribonucleotides Polymerase Chain Reaction RNA/genetics,isolation & purification Restriction Mapping Sequence Homology, Nucleic Acid Telencephalon/physiology
Chemicals
Codon Oligodeoxyribonucleotides RNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Murtha M T
Department of Biology, Yale University, New Haven, CT 06511.
Leckman J F
Ruddle F H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.23.10711
Published
1991-12-01
Pages
10711-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53000
Subset
IM
Grants
NIGMS NIH HHS · GM09966 · United States
NICHD NIH HHS · HD07412 · United States
Databases
GENBANK
M80723, M80724, M81658, M81659, M81660, M81661, M81662, M81663, M81664, M81665, M81666
External Links
PubMed source DOI: 10.1073/pnas.88.23.10711 PMC Full Text View journal details
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