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PMID: 17211577 Published · ppublish English Journal Article

An approach to the validation of novel molecular markers of breast cancer via TMA-based FISH scanning.

Journal of molecular histology ·Vol. 38 ·No. 2 ·2007-05-00 ·Pages 141-50

Tubbs RR, Swain E, Pettay JD, Hicks DG

Abstract

Tissue microarrays (TMA) are valuable tools for validating results of array-based comparative genomic hybridization (ACGH) and other translational research applications requiring independent verification of genomic gains and losses by fluorescence in situ hybridization (FISH). However, spatial orientation and accurate manual tracking of the TMA cores is challenging and prone to error. Image analysis combined with core tracking software, implemented via an automated FISH scanning workstation, represents a new approach to FISH and TMA-based validation of novel genomic changes discovered by ACGH in breast and other cancers. Automated large-scale tissue microarray validation FISH studies of genomic gains and losses identified by ACGH for breast cancer are feasible using an automated imaging scanner and tracking/classifying software. Furthermore, by leveraging the bifunctional fluorescent and chromogenic properties of the alkaline phosphatase chromogen fast red K and combining the technology with FISH, correlative and simultaneous phenotype/genotype studies may be enabled.

MeSH Terms
Biomarkers, Tumor/genetics Breast Neoplasms/diagnosis,genetics Centromere/genetics Chromosomes, Human, Pair 17/genetics Genotype Humans In Situ Hybridization, Fluorescence/instrumentation,methods Nucleic Acid Hybridization Phenotype Receptor, ErbB-2/genetics Tissue Array Analysis/instrumentation,methods
Chemicals
Biomarkers, Tumor Receptor, ErbB-2
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tubbs Raymond R
Departments of Anatomical and Clinical Pathology, The Cleveland Clinic Foundation, The Lerner College of Medicine, Cleveland, OH 44195, USA. [email protected]
Swain Eric
Pettay James D
Hicks David G
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Article Info
Journal
Journal of molecular histology
Abbr.
J Mol Histol
ISSN
1567-2379
DOI
10.1007/s10735-006-9076-z
Email
[email protected]
Published
2007-05-00
Epub
2007-00-09
Pages
141-50
Language
English
Region
Netherlands
NLM ID
101193653
Subset
IM
External Links
PubMed source DOI: 10.1007/s10735-006-9076-z View journal details
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