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PMID: 17214986 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Co-evolution of the archaeal tRNA-dependent amidotransferase GatCAB with tRNA(Asn).

FEBS letters ·Vol. 581 ·No. 2 ·2007-01-23 ·Pages 309-14

Namgoong S, Sheppard K, Sherrer RL, Söll D

Abstract

The important identity elements in tRNA(Gln) and tRNA(Asn) for bacterial GatCAB and in tRNA(Gln) for archaeal GatDE are the D-loop and the first base pair of the acceptor stem. Here we show that Methanothermobacter thermautotrophicus GatCAB, the archaeal enzyme, is different as it discriminates Asp-tRNA(Asp) and Asp-tRNA(Asn) by use of U49, the D-loop and to a lesser extent the variable loop. Since archaea possess the tRNA(Gln)-specific amidotransferase GatDE, the archaeal GatCAB enzyme evolved to recognize different elements in tRNA(Asn) than those recognized by GatDE or by the bacterial GatCAB enzyme in their tRNA substrates.

MeSH Terms
Archaeal Proteins/chemistry Evolution, Molecular Methanobacteriaceae/enzymology Nucleic Acid Conformation RNA, Archaeal/chemistry RNA, Transfer, Amino Acyl/chemistry RNA, Transfer, Asn/chemistry RNA, Transfer, Gln/chemistry Transaminases/chemistry
Chemicals
Archaeal Proteins RNA, Archaeal RNA, Transfer, Amino Acyl RNA, Transfer, Asn RNA, Transfer, Gln Transaminases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Namgoong Suk
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA.
Sheppard Kelly
Sherrer R Lynn
Söll Dieter
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Article Info
Journal
FEBS letters
Abbr.
FEBS Lett
ISSN
0014-5793
DOI
10.1016/j.febslet.2006.12.033
Published
2007-01-23
Epub
2007-00-02
Pages
309-14
Language
English
Region
England
NLM ID
0155157
PMCID
PMC1808439
Subset
IM
Grants
NIGMS NIH HHS · R01 GM022854 · United States
NIGMS NIH HHS · R37 GM022854 · United States
NIGMS NIH HHS · GM22854 · United States
External Links
PubMed source DOI: 10.1016/j.febslet.2006.12.033 PMC Full Text View journal details
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