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PMID: 9342321 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 22 ·1997-10-28 ·Pages 11819-26

Curnow AW, Hong Kw, Yuan R, Kim Si, Martins O, Winkler W, Henkin TM, Söll D

Abstract

The three genes, gatC, gatA, and gatB, which constitute the transcriptional unit of the Bacillus subtilis glutamyl-tRNAGln amidotransferase have been cloned. Expression of this transcriptional unit results in the production of a heterotrimeric protein that has been purified to homogeneity. The enzyme furnishes a means for formation of correctly charged Gln-tRNAGln through the transamidation of misacylated Glu-tRNAGln, functionally replacing the lack of glutaminyl-tRNA synthetase activity in Gram-positive eubacteria, cyanobacteria, Archaea, and organelles. Disruption of this operon is lethal. This demonstrates that transamidation is the only pathway to Gln-tRNAGln in B. subtilis and that glutamyl-tRNAGln amidotransferase is a novel and essential component of the translational apparatus.

MeSH Terms
Acylation Amino Acid Sequence Bacillus subtilis/enzymology,genetics Base Sequence Codon Escherichia coli/genetics Glutamine/genetics Molecular Sequence Data Mutagenesis, Insertional Nitrogenous Group Transferases/genetics,isolation & purification,metabolism Protein Biosynthesis Recombinant Proteins/genetics,isolation & purification Sequence Homology, Amino Acid Substrate Specificity
Chemicals
Codon Recombinant Proteins Glutamine Nitrogenous Group Transferases glutamyl-tRNA(Gln) amidotransferase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Curnow A W
Department of Molecular Biophysics, Yale University, New Haven, CT 06520-8114, USA.
Hong K w
Yuan R
Kim S i
Martins O
Winkler W
Henkin T M
Söll D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.22.11819
Published
1997-10-28
Pages
11819-26
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC23611
Subset
IM
Databases
GENBANK
AF008553
Corrections
CommentIn
PMID: 9342308
External Links
PubMed source DOI: 10.1073/pnas.94.22.11819 PMC Full Text View journal details
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