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PMID: 1723106 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of a DNA region comprising the gene for elongation factor 1 alpha (EF-1 alpha) from the ultrathermophilic archaeote Pyrococcus woesei: phylogenetic implications.

Journal of molecular evolution ·Vol. 33 ·No. 4 ·1991-10-00 ·Pages 332-42

Creti R, Citarella F, Tiboni O, Sanangelantoni A, Palm P, Cammarano P

Abstract

The gene encoding elongation factor 1 alpha (EF-1 alpha, 1290 bp) of the ultrathermophilic, sulfur-reducing archaeote Pyrococcus woesei was localized within a Bg/II fragment of chromosomal DNA. Sequence analysis showed that the EF-1 alpha gene is the upstream unit of a three-gene cluster comprising the genes for ribosomal protein S10 (306 bp) and transfer RNAser (GGA). The three genes follow each other immediately in the order EF-1 alpha.S10.tRNA(ser) after a putative promoter located 55 bp upstream of the EF-1 alpha gene. Alignment of the derived EF-1 alpha sequence with the corresponding sequences from Eukarya, Bacteria/organelles, and with available archaeal sequences (Sulfolobus, Thermococcus, Methanococcus, Halobacterium) showed that Pyrococcus EF-1 alpha is highly homologous (89% identity) to Thermococcus celer EF-1 alpha, both being strikingly more similar to eukaryotic EF-1 alpha than to bacterial EF-Tu. Unrooted dendrograms computed from aligned sequences by distance matrix and DNA parsimony methods, including evolutionary parsimony, showed the Archaea to be a monophyletic-holophyletic cluster closer to Eukarya than to Bacteria. Both distance matrix and DNA parsimony--although not evolutionary parsimony--support the partition of the known archaeal lineages between the kingdoms Crenarchaeota and Euryarchaeota, and the affiliation of the Pyrococcus-Thermococcus lineage to the Euryarchaeota, of which it is the most primitive offspring. A closer relation of Pyrococcus to Euryarchaeota than to Crenarchaeota was also inferred from sequence analysis of S10 ribosomal proteins.

Related Genes
EF-1&agr
MeSH Terms
Amino Acid Sequence Archaea/classification,genetics Base Sequence Cloning, Molecular DNA, Bacterial Genetic Linkage Molecular Sequence Data Peptide Elongation Factor 1 Peptide Elongation Factor Tu/genetics Peptide Elongation Factors/genetics Phylogeny RNA, Bacterial Ribosomal Proteins/genetics Sequence Alignment
Chemicals
DNA, Bacterial Peptide Elongation Factor 1 Peptide Elongation Factors RNA, Bacterial Ribosomal Proteins ribosomal protein S10 Peptide Elongation Factor Tu
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Creti R
Dipartimento di Biopatologia Umana, Università di Roma La Sapienza, Policlinico Umberto I., Italy.
Citarella F
Tiboni O
Sanangelantoni A
Palm P
Cammarano P
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Article Info
Journal
Journal of molecular evolution
Abbr.
J Mol Evol
ISSN
0022-2844
DOI
10.1007/BF02102864
Published
1991-10-00
Pages
332-42
Language
English
Region
Germany
NLM ID
0360051
Subset
IM
Databases
GENBANK
S77967, X59857, X63302, X63303, X63304, X63305, X63306, X63307, X63308, X63309
External Links
PubMed source DOI: 10.1007/BF02102864 View journal details
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