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PMID: 1723915 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nitric oxide synthase in cultured endocardial cells of the pig.

British journal of pharmacology ·Vol. 104 ·No. 1 ·1991-09-00 ·Pages 21-4

Schulz R, Smith JA, Lewis MJ, Moncada S

Abstract

1. Endocardial cells release factors which regulate myocardial contractility and guanosine 3':5'-cyclic monophosphate (cyclic GMP) levels. One of these factors is indistinguishable from endothelium-derived relaxing factor (EDRF). 2. The effluent from pig heart endocardial cells cultured on microcarrier beads caused the relaxation of a pig coronary artery ring denuded of endothelium. This relaxation was enhanced by a combination of superoxide dismutase and catalase and was attenuated by haemoglobin, which binds nitric oxide (NO), and by inhibitors of NO synthase, NG-monomethyl-L-arginine (L-NMMA) or NG-nitro-L-arginine. 3. A Ca(2+)-, L-arginine- and NADPH-dependent enzyme activity which generated NO was detected by a specific spectrophotometric assay in cytosol prepared from endocardial cells. The formation of NO was inhibited in a concentration-dependent manner by L-NMMA (but not D-NMMA) and this could be partially reversed upon addition of excess L-arginine. 4. Like endothelial cells from the blood vessels, the endocardial cells possess the ability to synthesize NO, which may act to regulate myocardial contractility.

MeSH Terms
Amino Acid Oxidoreductases/metabolism Animals Arginine/analogs & derivatives,metabolism Calcium/physiology Cells, Cultured Cytosol/enzymology Endocardium/metabolism Nitric Oxide/metabolism Nitric Oxide Synthase Swine omega-N-Methylarginine
Chemicals
omega-N-Methylarginine Nitric Oxide Arginine Nitric Oxide Synthase Amino Acid Oxidoreductases Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schulz R
Wellcome Research Laboratories, Beckenham, Kent.
Smith J A
Lewis M J
Moncada S
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Article Info
Journal
British journal of pharmacology
Abbr.
Br J Pharmacol
ISSN
0007-1188
DOI
10.1111/j.1476-5381.1991.tb12378.x
Published
1991-09-00
Pages
21-4
Language
English
Region
England
NLM ID
7502536
PMCID
PMC1908256
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1476-5381.1991.tb12378.x PMC Full Text View journal details
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