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PMID: 172638 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Distribution of membrane-bound cyclic AMP-dependent protein kinase in plasma membranes of cells of the kidney cortex.

The Journal of membrane biology ·Vol. 24 ·No. 2 ·1975-11-07 ·Pages 145-59

Kinne R, Shlatz LJ, Kinne-Saffran E, Schwartz IL

Abstract

Renal cortical plasms membranes were separated by free flow electrophoresis into luminal (brush border microvilli) and contraluminal (basal-lateral membrane) fractions. These membranes were found to contain an intrinsic, self-phosphorylating system which consists of a cyclic AMP-dependent protein kinase, a phosphorprotein phosphatase and the substrate(s) of these enzymes. The kinase, but not the phosphatase, was stimulated by cyclic AMP; maximal (1.7-fold) stimulation was effected at a cyclic AMP concentration of 0.1 muM. The degree of phosphorylation of the brush borders was six times greater than that of the basal-lateral membranes in the absence of cyclic AMP and 2.3-fold greater in the presence of cyclic AMP. This preferential phosphorylation of the luminal membrane by membrane-associated protein kinase(s) may play a role in the parathyroid hormone-mediated alterations of solute reabsorption in the proximal tubule.

MeSH Terms
Adenosine Triphosphatases/metabolism Adenosine Triphosphate/pharmacology Alkaline Phosphatase/metabolism Animals Cell Membrane/drug effects,enzymology Cyclic AMP/pharmacology Cytosol/enzymology Enzyme Activation/drug effects Kidney Cortex/cytology,enzymology Kinetics Magnesium/pharmacology Ouabain/pharmacology Potassium/pharmacology Potassium Chloride/pharmacology Protein Binding Protein Kinases/metabolism Rats Sodium/pharmacology
Chemicals
Ouabain Potassium Chloride Adenosine Triphosphate Sodium Cyclic AMP Protein Kinases Alkaline Phosphatase Adenosine Triphosphatases Magnesium Potassium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kinne R
Shlatz L J
Kinne-Saffran E
Schwartz I L
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43 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01868620
Published
1975-11-07
Pages
145-59
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01868620 View journal details
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