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PMID: 17264999 Published · ppublish English Comparative Study Journal Article

Species-specific bacterial communities in the phycosphere of microalgae?

Microbial ecology ·Vol. 53 ·No. 4 ·2007-05-00 ·Pages 683-99

Sapp M, Schwaderer AS, Wiltshire KH, Hoppe HG, Gerdts G, Wichels A

Abstract

Specific associations of bacteria with phytoplankton have recently been reported in the literature. In our study, we analyzed bacterial communities of microalgal cultures related to algal growth phases. Seven freshly isolated key diatom and dinoflagellate species from Helgoland Roads, North Sea, were investigated. The community composition of associated bacteria as well as the cell numbers, the photosynthetic efficiency of the algae, and the depletion of inorganic nutrients in the medium were recorded over a period of 8 weeks in batch cultures. Diversity and succession of bacterial communities was analyzed by ribosomal intergenic spacer analysis. Phylogenetic analysis of bacterial populations was performed by denaturing gradient gel electrophoresis of 16S rRNA genes followed by DNA sequence analysis. Members of Alphaproteobacteria and Gammaproteobacteria and the Flavobacteria-Sphingobacteria group within the Bacteroidetes phylum predominated in the cultures. Differences in free-living and attached bacterial populations were observed between the phylogenetic groups. Shifts in the bacterial communities could not be correlated to changes of nutrient levels or algal growth phases. Regarding our results, it should not be generalized that the compositions of the bacterial communities are strictly species specific for microalgae. The importance of factors like the composition of exudates is apparent.

MeSH Terms
Animals Bacteroidetes/classification,genetics,physiology Cluster Analysis DNA, Ribosomal Spacer/chemistry Diatoms/growth & development,microbiology Dinoflagellida/growth & development,microbiology North Sea Photosynthesis/physiology Phylogeny Proteobacteria/classification,genetics,physiology Sequence Analysis, DNA Species Specificity Water Microbiology
Chemicals
DNA, Ribosomal Spacer
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sapp Melanie
Alfred Wegener Institute Foundation for Polar and Marine Research, Biologische Anstalt Helgoland, Helgoland, Germany. [email protected]
Schwaderer Anne S
Wiltshire Karen H
Hoppe Hans-Georg
Gerdts Gunnar
Wichels Antje
References (32)
32 references, click to expand
  1. Plankton diversity in the Bay of Fundy as measured by morphological and molecular methods.
    Microb Ecol. 2004 Jul;48(1):51-65 PMID: 15164237
  2. Bacterial primary colonization and early succession on surfaces in marine waters as determined by amplified rRNA gene restriction analysis and sequence analysis of 16S rRNA genes.
    Appl Environ Microbiol. 2000 Feb;66(2):467-75 PMID: 10653705
  3. Dynamics of bacterial community composition and activity during a mesocosm diatom bloom.
    Appl Environ Microbiol. 2000 Feb;66(2):578-87 PMID: 10653721
  4. Relationship between bacterial community composition and bottom-up versus top-down variables in four eutrophic shallow lakes.
    Appl Environ Microbiol. 2002 Oct;68(10):4740-50 PMID: 12324315
  5. ARB: a software environment for sequence data.
    Nucleic Acids Res. 2004 Feb 25;32(4):1363-71 PMID: 14985472
  6. Within- and between-lake variability in the composition of bacterioplankton communities: investigations using multiple spatial scales.
    Appl Environ Microbiol. 2004 Jan;70(1):214-23 PMID: 14711644
  7. A molecular phylogenetic survey of sea-ice microbial communities (SIMCO).
    FEMS Microbiol Ecol. 2001 May;35(3):267-275 PMID: 11311437
  8. Temporal patterns in bacterial communities in three temperate lakes of different trophic status.
    Microb Ecol. 2003 Nov;46(4):391-405 PMID: 12904915
  9. Key aromatic-ring-cleaving enzyme, protocatechuate 3,4-dioxygenase, in the ecologically important marine Roseobacter lineage.
    Appl Environ Microbiol. 2000 Nov;66(11):4662-72 PMID: 11055908
  10. Heterogeneous Cell Density and Genetic Structure of Bacterial Pools Associated with Various Soil Microenvironments as Determined by Enumeration and DNA Fingerprinting Approach (RISA).
    Microb Ecol. 2000 May;39(4):263-272 PMID: 10882431
  11. Genetic diversity of 'satellite' bacteria present in cultures of marine diatoms.
    FEMS Microbiol Ecol. 2002 Oct 1;42(1):25-35 PMID: 19709263
  12. Natural assemblages of marine proteobacteria and members of the Cytophaga-Flavobacter cluster consuming low- and high-molecular-weight dissolved organic matter.
    Appl Environ Microbiol. 2000 Apr;66(4):1692-7 PMID: 10742262
  13. Algae acquire vitamin B12 through a symbiotic relationship with bacteria.
    Nature. 2005 Nov 3;438(7064):90-3 PMID: 16267554
  14. Successional changes in the genetic diversity of a marine bacterial assemblage during confinement.
    Arch Microbiol. 2000 Feb;173(2):138-45 PMID: 10795685
  15. Links between phytoplankton and bacterial community dynamics in a coastal marine environment.
    Microb Ecol. 2005 Jan;49(1):163-75 PMID: 15688258
  16. Culturability and In situ abundance of pelagic bacteria from the North Sea.
    Appl Environ Microbiol. 2000 Jul;66(7):3044-51 PMID: 10877804
  17. Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology.
    Antonie Van Leeuwenhoek. 1998 Jan;73(1):127-41 PMID: 9602286
  18. Algicidal effects of a novel marine pseudoalteromonas isolate (class Proteobacteria, gamma subdivision) on harmful algal bloom species of the genera Chattonella, Gymnodinium, and Heterosigma.
    Appl Environ Microbiol. 1998 Aug;64(8):2806-13 PMID: 9687434
  19. The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy.
    Nucleic Acids Res. 2003 Jan 1;31(1):442-3 PMID: 12520046
  20. Phylogenetic and functional diversity of the cultivable bacterial community associated with the paralytic shellfish poisoning dinoflagellate Gymnodinium catenatum.
    FEMS Microbiol Ecol. 2004 Mar 1;47(3):345-57 PMID: 19712323
  21. Isolation of novel pelagic bacteria from the German bight and their seasonal contributions to surface picoplankton.
    Appl Environ Microbiol. 2001 Nov;67(11):5134-42 PMID: 11679337
  22. Differential growth response of colony-forming alpha- and gamma-proteobacteria in dilution culture and nutrient addition experiments from Lake Kinneret (Israel), the eastern Mediterranean Sea, and the Gulf of Eilat.
    Appl Environ Microbiol. 2003 Jan;69(1):199-211 PMID: 12513996
  23. Response of marine bacterioplankton to differential filtration and confinement.
    Appl Environ Microbiol. 1984 Jan;47(1):49-55 PMID: 6696422
  24. Numerical dominance of a group of marine bacteria in the alpha-subclass of the class Proteobacteria in coastal seawater.
    Appl Environ Microbiol. 1997 Nov;63(11):4237-42 PMID: 9361410
  25. Phylogenetic diversity and specificity of bacteria closely associated with Alexandrium spp. and other phytoplankton.
    Appl Environ Microbiol. 2005 Jul;71(7):3483-94 PMID: 16000752
  26. Electrophoresis time impacts the denaturing gradient gel electrophoresis-based assessment of bacterial community structure.
    J Microbiol Methods. 2004 Apr;57(1):17-22 PMID: 15003684
  27. Bacteria of the Genus Roseobacter Associated with the Toxic Dinoflagellate Prorocentrum lima.
    Protist. 1998 Dec;149(4):347-57 PMID: 23194717
  28. Regulation of oceanic silicon and carbon preservation by temperature control on bacteria.
    Science. 2002 Dec 6;298(5600):1980-4 PMID: 12471255
  29. Marine diatom species harbour distinct bacterial communities.
    Environ Microbiol. 2005 Jun;7(6):860-73 PMID: 15892705
  30. Aequorivita gen. nov., a member of the family Flavobacteriaceae isolated from terrestrial and marine Antarctic habitats.
    Int J Syst Evol Microbiol. 2002 Sep;52(Pt 5):1533-41 PMID: 12361255
  31. Sequencing bands of ribosomal intergenic spacer analysis fingerprints for characterization and microscale distribution of soil bacterium populations responding to mercury spiking.
    Appl Environ Microbiol. 2000 Dec;66(12):5334-9 PMID: 11097911
  32. Pseudoalteromonas agarivorans sp. nov., a novel marine agarolytic bacterium.
    Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):125-31 PMID: 12656163
Article Info
Journal
Microbial ecology
Abbr.
Microb Ecol
ISSN
0095-3628
DOI
10.1007/s00248-006-9162-5
Email
[email protected]
Published
2007-05-00
Epub
2007-00-31
Pages
683-99
Language
English
Region
United States
NLM ID
7500663
Subset
IM
External Links
PubMed source DOI: 10.1007/s00248-006-9162-5 View journal details
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