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PMID: 1729218 Published · ppublish English Journal Article

Sequence and expression of the Escherichia coli K1 neuC gene product.

Journal of bacteriology ·Vol. 174 ·No. 1 ·1992-01-00 ·Pages 315-9

Zapata G, Crowley JM, Vann WF

Abstract

The nucleotide sequence of the neuC gene of the Escherichia coli K1 capsule gene cluster encodes a protein with a predicted molecular weight of 44,210 containing 391 amino acids. A chimeric protein with beta-galactosidase fused to the carboxy terminus of the neuC gene product (P7) was constructed and purified. Its amino-terminal sequence confirmed the prediction from the nucleotide sequence that the neuC gene overlaps the distal end of the neuA gene by a single base pair. Both the neuA and neuC genes are coexpressed under the control of a single upstream T7 or tac promoter, suggesting that neuA and neuC are part of an operon.

Related Genes
neuC
MeSH Terms
Amino Acid Sequence Antigens, Bacterial/genetics Antigens, Surface/genetics Bacterial Capsules/genetics Base Sequence Cloning, Molecular Escherichia coli/genetics Gene Expression Regulation, Bacterial Lac Operon Molecular Sequence Data N-Acetylneuraminic Acid N-Acylneuraminate Cytidylyltransferase/genetics Promoter Regions, Genetic Recombinant Fusion Proteins Sialic Acids/genetics
Chemicals
Antigens, Bacterial Antigens, Surface K antigens Recombinant Fusion Proteins Sialic Acids N-Acylneuraminate Cytidylyltransferase N-Acetylneuraminic Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zapata G
Laboratory of Bacterial Polysaccharides, Center for Biologics Evaluation and Research, Bethesda, Maryland 20892.
Crowley J M
Vann W F
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.1.315-319.1992
Published
1992-01-00
Pages
315-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205711
Subset
IM
Databases
GENBANK
M79367, M79368, M79369, M79370, M79371, M79372, M84026, M87491, M87492, M87888
External Links
PubMed source DOI: 10.1128/jb.174.1.315-319.1992 PMC Full Text View journal details
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