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PMID: 1729607 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two conserved essential motifs of the murine immunoglobulin lambda enhancers bind B-cell-specific factors.

Molecular and cellular biology ·Vol. 12 ·No. 1 ·1992-01-00 ·Pages 309-20

Rudin CM, Storb U

Abstract

Two highly homologous enhancers associated with the two murine immunoglobulin lambda constant-region clusters were recently identified. In order to better understand the molecular basis for the developmental stage- and cell-type-restricted expression of lambda genes, we have undertaken an analysis of the putative regulatory domains of these enhancers. By using a combination of DNase I footprinting, electrophoretic mobility shift assay, and site-specific mutations, four candidate protein binding sites have been identified at analogous positions in both enhancers. A mutation of any of these sites decreases enhancer activity. Two of the sites, lambda A and lambda B, are essential for enhancer function, and both of these sites appear to bind both B-cell-specific and general factors. Nevertheless, isolated lambda A and lambda B sites show no evidence of inherent transactivating potential, alone or together, even when present in up to three copies. We suggest that the generation of transactivating signals from these enhancers may require the complex interaction of multiple B-cell-specific and nonspecific DNA-binding factors.

MeSH Terms
Animals B-Lymphocytes/metabolism Base Sequence Binding Sites DNA Deoxyribonuclease I/metabolism Enhancer Elements, Genetic Gene Expression Regulation Immunoglobulin Light Chains/genetics Immunoglobulin lambda-Chains/genetics Macromolecular Substances Mice Molecular Sequence Data Mutation Transcription Factors/metabolism Transfection Tumor Cells, Cultured
Chemicals
Immunoglobulin Light Chains Immunoglobulin lambda-Chains Macromolecular Substances Transcription Factors DNA Deoxyribonuclease I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rudin C M
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Storb U
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.1.309-320.1992
Published
1992-01-00
Pages
309-20
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364111
Subset
IM
Grants
NICHD NIH HHS · HD07136 · United States
External Links
PubMed source DOI: 10.1128/mcb.12.1.309-320.1992 PMC Full Text View journal details
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