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PMID: 1730751 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Perturbation of the morphology of the trans-Golgi network following Brefeldin A treatment: redistribution of a TGN-specific integral membrane protein, TGN38.

The Journal of cell biology ·Vol. 116 ·No. 1 ·1992-01-00 ·Pages 85-94

Reaves B, Banting G

Abstract

Brefeldin A (BFA) has a dramatic effect on the morphology of the Golgi apparatus and induces a rapid redistribution of Golgi proteins into the ER (Lippincott-Schwartz, J., L. C. Yuan, J. S. Bonifacino, and R. D. Klausner. 1989. Cell. 56:801-813). To date, no evidence that BFA affects the morphology of the trans-Golgi network (TGN) has been presented. We describe the results of experiments, using a polyclonal antiserum to a TGN specific integral membrane protein (TGN38) (Luzio, J.P., B. Brake, G. Banting, K. E. Howell, P. Braghetta, and K. K. Stanley. 1990. Biochem. J. 270:97-102), which demonstrate that incubation of cells with BFA does induce morphological changes to the TGN. However, rather than redistributing to the ER, the majority of the TGN collapses around the microtubule organizing center (MTOC). The effect of BFA upon the TGN is (a) independent of protein synthesis, (b) fully reversible (c) microtubule dependent (as shown in nocodazole-treated cells), and (d) relies upon the hydrolysis of GTP (as shown by performing experiments in the presence of GTP gamma S). ATP depletion reduces the ability of BFA to induce a redistribution of Golgi proteins into the ER; however, it has no effect upon the BFA-induced relocalizations of the TGN. These data confirm that the TGN is an organelle which is independent of the Golgi, and suggest a dynamic interaction between the TGN and microtubules which is centered around the MTOC.

MeSH Terms
Animals Anti-Bacterial Agents/pharmacology Brefeldin A Cells, Cultured Cyclopentanes/pharmacology Fluorescent Antibody Technique Glycoproteins Golgi Apparatus/drug effects,ultrastructure Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology Guanosine Triphosphate/metabolism Kidney Membrane Glycoproteins/metabolism Membrane Proteins/analysis,metabolism Nocodazole/pharmacology Rats
Chemicals
Anti-Bacterial Agents Cyclopentanes Glycoproteins Membrane Glycoproteins Membrane Proteins Tgoln2 protein, rat Brefeldin A Guanosine 5'-O-(3-Thiotriphosphate) Guanosine Triphosphate Nocodazole
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Reaves B
Department of Biochemistry, School of Medical Sciences, University of Bristol, U.K.
Banting G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.116.1.85
Published
1992-01-00
Pages
85-94
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289270
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.116.1.85 PMC Full Text View journal details
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