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PMID: 17312019 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Defects in lamin B1 expression or processing affect interphase chromosome position and gene expression.

The Journal of cell biology ·Vol. 176 ·No. 5 ·2007-02-26 ·Pages 593-603

Malhas A, Lee CF, Sanders R, Saunders NJ, Vaux DJ

Abstract

Radial organization of nuclei with peripheral gene-poor chromosomes and central gene-rich chromosomes is common and could depend on the nuclear boundary as a scaffold or position marker. To test this, we studied the role of the ubiquitous nuclear envelope (NE) component lamin B1 in NE stability, chromosome territory position, and gene expression. The stability of the lamin B1 lamina is dependent on lamin endoproteolysis (by Rce1) but not carboxymethylation (by Icmt), whereas lamin C lamina stability is not affected by the loss of full-length lamin B1 or its processing. Comparison of wild-type murine fibroblasts with fibroblasts lacking full-length lamin B1, or defective in CAAX processing, identified genes that depend on a stable processed lamin B1 lamina for normal expression. We also demonstrate that the position of mouse chromosome 18 but not 19 is dependent on such a stable nuclear lamina. The results implicate processed lamin B1 in the control of gene expression as well as chromosome position.

MeSH Terms
Animals Cell Compartmentation Chromosomes, Mammalian Endopeptidases/metabolism Gene Expression Profiling Gene Expression Regulation Interphase/genetics,physiology Lamin Type B/genetics,metabolism Mice Nuclear Lamina/genetics,metabolism,ultrastructure Oligonucleotide Array Sequence Analysis Protein Processing, Post-Translational/physiology
Chemicals
Lamin Type B lamin B1 Endopeptidases Rce1 protein, mouse
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Malhas Ashraf
Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, England, UK.
Lee Chiu Fan
Sanders Rebecca
Saunders Nigel J
Vaux David J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200607054
Published
2007-02-26
Epub
2007-00-20
Pages
593-603
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2064018
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200607054 PMC Full Text View journal details
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