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PMID: 17344476 Published · ppublish English Journal Article

The transcriptional repressor Glis2 is a novel binding partner for p120 catenin.

Molecular biology of the cell ·Vol. 18 ·No. 5 ·2007-05-00 ·Pages 1918-27

Hosking CR, Ulloa F, Hogan C, Ferber EC, Figueroa A, Gevaert K, Birchmeier W, Briscoe J, Fujita Y

Abstract

In epithelial cells, p120 catenin (p120) localizes at cell-cell contacts and regulates adhesive function of the cadherin complex. In addition, p120 has been reported to localize in the nucleus, although the nuclear function of p120 is not fully understood. Here, we report the identification of Gli-similar 2 (Glis2) as a novel binding protein for p120. Glis2 is a Krüppel-like transcriptional repressor with homology to the Gli family, but its physiological function has not been well characterized. In this study, we show that coexpression of Glis2 and Src induces nuclear translocation of p120. Furthermore, p120 induces the C-terminal cleavage of Glis2, and this cleavage is further enhanced by Src. The cleaved form of Glis2 loses one of its five zinc finger domains, but it is still able to bind DNA. Functional studies in chick neural tube indicate that full-length Glis2 can affect neuronal differentiation, whereas the cleaved form requires coexpression of p120 to have a similar effect. These data indicate that p120 has additional novel functions in the nucleus together with Glis2.

MeSH Terms
Active Transport, Cell Nucleus Animals COS Cells Catenins Cattle Cell Adhesion Molecules/genetics,metabolism Cell Differentiation Cell Line Chick Embryo Chlorocebus aethiops DNA/metabolism Humans In Vitro Techniques Kruppel-Like Transcription Factors/chemistry,genetics,metabolism Neurons/cytology,metabolism Phosphoproteins/genetics,metabolism Protein Binding RNA Interference Recombinant Fusion Proteins/chemistry,genetics,metabolism Two-Hybrid System Techniques Zinc Fingers src-Family Kinases/metabolism
Chemicals
Catenins Cell Adhesion Molecules GLIS2 protein, human Kruppel-Like Transcription Factors Phosphoproteins Recombinant Fusion Proteins delta catenin DNA src-Family Kinases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Hosking Catherine Rose
Medical Research Council Laboratory for Molecular Cell Biology and Cell Biology Unit, Department of Biology, University College London, London WC1E 6BT, UK.
Ulloa Fausto
Hogan Catherine
Ferber Emma C
Figueroa Angélica
Gevaert Kris
Birchmeier Walter
Briscoe James
Fujita Yasuyuki
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e06-10-0941
Published
2007-05-00
Epub
2007-00-07
Pages
1918-27
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1855037
Subset
IM
Grants
Medical Research Council · MC_U117560541 · United Kingdom
Medical Research Council · MC_U122669938 · United Kingdom
External Links
PubMed source DOI: 10.1091/mbc.e06-10-0941 PMC Full Text View journal details
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