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PMID: 174111 Published · ppublish English Journal Article

Effect of Xenopus laevis oocyte extract on supercoiled simian virus 40 DNA: formation of complex DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 2 ·1976-02-00 ·Pages 554-8

Gandini Attardi D, Martini G, Mattoccia E, Tocchini-Valentini GP

Abstract

A soluble cell-free extract derived from stage 6 Xenopus laevis oocytes is described. From supercoiled simian virus 40 DNA the extract produces nicked circles (having a single-strand scission), linear molecules of full unit size, shorter length fragments, and various forms of complex DNA.

MeSH Terms
Animals Cell Nucleus/metabolism Cell-Free System Cytoplasm/metabolism DNA Replication DNA, Circular/metabolism DNA, Viral/metabolism Deoxyribonucleotides/metabolism Female Guanine Nucleotides/metabolism Microscopy, Electron Nucleic Acid Conformation Oocytes/metabolism,ultrastructure Ovum/metabolism Simian virus 40 Xenopus
Chemicals
DNA, Circular DNA, Viral Deoxyribonucleotides Guanine Nucleotides
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gandini Attardi D
Martini G
Mattoccia E
Tocchini-Valentini G P
References (18)
18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.2.554
Published
1976-02-00
Pages
554-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC335948
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.2.554 PMC Full Text View journal details
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