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PMID: 17438077 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A signal from inside the peroxisome initiates its division by promoting the remodeling of the peroxisomal membrane.

The Journal of cell biology ·Vol. 177 ·No. 2 ·2007-04-23 ·Pages 289-303

Guo T, Gregg C, Boukh-Viner T, Kyryakov P, Goldberg A, Bourque S, Banu F, Haile S, Milijevic S, San KH, Solomon J, Wong V, Titorenko VI

Abstract

We define the dynamics of spatial and temporal reorganization of the team of proteins and lipids serving peroxisome division. The peroxisome becomes competent for division only after it acquires the complete set of matrix proteins involved in lipid metabolism. Overloading the peroxisome with matrix proteins promotes the relocation of acyl-CoA oxidase (Aox), an enzyme of fatty acid beta-oxidation, from the matrix to the membrane. The binding of Aox to Pex16p, a membrane-associated peroxin required for peroxisome biogenesis, initiates the biosynthesis of phosphatidic acid and diacylglycerol (DAG) in the membrane. The formation of these two lipids and the subsequent transbilayer movement of DAG initiate the assembly of a complex between the peroxins Pex10p and Pex19p, the dynamin-like GTPase Vps1p, and several actin cytoskeletal proteins on the peroxisomal surface. This protein team promotes membrane fission, thereby executing the terminal step of peroxisome division.

MeSH Terms
Acyl-CoA Oxidase/metabolism Acyltransferases/metabolism Cytosol/chemistry Diglycerides/metabolism Endoplasmic Reticulum/chemistry Fungal Proteins/metabolism Intracellular Membranes/chemistry,metabolism Membrane Proteins/metabolism Multiprotein Complexes/metabolism Peroxisomes/chemistry,metabolism Phosphatidate Phosphatase/metabolism Phosphatidic Acids/metabolism Phosphatidylcholines/metabolism Signal Transduction Yarrowia/metabolism,ultrastructure
Chemicals
Diglycerides Fungal Proteins Membrane Proteins Multiprotein Complexes PEX16 protein, Yarrowia lipolytica Phosphatidic Acids Phosphatidylcholines Acyl-CoA Oxidase Acyltransferases 2-acylglycerophosphate acyltransferase Phosphatidate Phosphatase
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Guo Tong
Department of Biology, Concordia University, Montreal, Quebec H4B 1R6, Canada.
Gregg Christopher
Boukh-Viner Tatiana
Kyryakov Pavlo
Goldberg Alexander
Bourque Simon
Banu Farhana
Haile Sandra
Milijevic Svetlana
San Karen Hung Yeung
Solomon Jonathan
Wong Vivianne
Titorenko Vladimir I
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200609072
Published
2007-04-23
Epub
2007-00-16
Pages
289-303
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2064137
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200609072 PMC Full Text View journal details
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