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PMID: 17439403 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Polycomb protein Cbx4 promotes SUMO modification of de novo DNA methyltransferase Dnmt3a.

The Biochemical journal ·Vol. 405 ·No. 2 ·2007-07-15 ·Pages 369-78

Li B, Zhou J, Liu P, Hu J, Jin H, Shimono Y, Takahashi M, Xu G

Abstract

The 'de novo methyltransferase' Dnmt3a (DNA methyltransferase 3a) has been shown to mediate transcriptional repression. Post-translational modification of Dnmt3a by SUMOylation affects its ability to transcriptionally repress. However, very little is known about how the SUMOylation process is regulated. In the present study, we identified a PcG (Polycomb group) protein, Cbx4 (chromobox 4), as a specific interaction partner of Dnmt3a. Co-expression of Cbx4 and SUMO-1 (small ubiquitin-related modifier-1) along with Dnmt3a in transfected cells results in enhanced modification of Dnmt3a with SUMO-1. Purified Cbx4 also promotes SUMOylation of Dnmt3a in vitro. The modification occurs in the N-terminal regulatory region, including the PWWP (Pro-Trp-Trp-Pro) domain. Our results suggest that Cbx4 functions as a SUMO E3 ligase for Dnmt3a and it might be involved in the functional regulation of DNA methyltransferases by promoting their SUMO modification.

MeSH Terms
Amino Acid Sequence Animals Cells, Cultured DNA (Cytosine-5-)-Methyltransferases/metabolism DNA Methylation DNA Methyltransferase 3A Heterochromatin/enzymology Humans Ligases Mice Molecular Sequence Data Polycomb-Group Proteins Protein Structure, Tertiary Repressor Proteins/physiology SUMO-1 Protein/metabolism Sequence Alignment Transfection Ubiquitin-Protein Ligases/metabolism
Chemicals
DNMT3A protein, human Dnmt3a protein, mouse Heterochromatin Polycomb-Group Proteins Repressor Proteins SUMO-1 Protein DNA (Cytosine-5-)-Methyltransferases DNA Methyltransferase 3A Ubiquitin-Protein Ligases Ligases CBX4 protein, human
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Li Bing
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, People's Republic of China.
Zhou Jing
Liu Peng
Hu Jialei
Jin Hong
Shimono Yohei
Takahashi Masahide
Xu Guoliang
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
1470-8728
DOI
10.1042/BJ20061873
Published
2007-07-15
Pages
369-78
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1904525
Subset
IM
External Links
PubMed source DOI: 10.1042/BJ20061873 PMC Full Text View journal details
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